1 The world leader in serving science Proprietary & Confidential WiSIM DIA Method Set-up Orbitrap Fusion MS MS Instrument Control Software v.1.1 SP1.

Slides:

Advertisements

Similar presentations

Details of Tune and Method Setup for LTQ Orbitrap

Advertisements

Forensic Drug Testing Part 2: GC/MS Confirmation

Agilent 6410 Triple Quad LC/MS Software

Protein Quantitation II: Multiple Reaction Monitoring

Orbitrap Mass Analyser - Overview and Applications in Proteomics

Performance of a Hybrid RF/DC Quadrupole-Linear Ion Trap Mass Spectrometer James W. Hager MDS SCIEX ASMS, 2002.

FC-MS from Teledyne Isco CombiFlash ® a Name You Can Rely On.

Improvements in Mass Spectrometry for Life Science Research – Does Agilent Have the Answer? Ashley Sage PhD.

Method Building Essentials

Mass Spectrometry Inlet system Ion Source Mass Analyzer Ion Detector.

With Supersonic Molecular Beams Maya Kochman and Aviv Amirav

Mass Analyzers Double Focusing Magnetic Sector Quadrupole Mass Filter Quadrupole Ion Trap Linear Time-of-Flight (TOF) Reflectron TOF Fourier Transform.

Mass Spectrometry. How is MS Done? Basic MS Instrumentation.

Mass Analyzers Double Focusing Magnetic Sector Quadrupole Mass Filter Quadrupole Ion Trap Linear Time-of-Flight (TOF) Reflectron TOF Fourier Transform.

Atomic Mass Spectrometry

LC/MS WORKSHOP IOWA STATE UNIVERSITY Kamel Harrata  Instrument Description  Data Acquisition  Data Processing.

Development of a TOF Version of the Desktop MiniSIMS Design & Applications A.J. Eccles, B. Cliff, C. Jones, N. Long, P. Vohralik Millbrook Instruments.

Advanced Electrophoresis Solutions Ltd

GCMS: gas chromatography and mass spectrometry

020219GKRECYCLE GC RECYCLE CHROMATOGRAPHY Jiri SEVCIK Prague, the Czech Republic.

Q-Exactive LC-MS. TurboFlow technology - Special columns - Complex valving - Extra pump UHPLC -Fast separation -Best separation.

Center for Human Health and the Environment

Atomic Theory (Or a quick Chemistry Review). Atomic Theory Q: What does science study? A: The natural world, the physical universe Q: What are the components.

Quantification Techniques Under Dynamic SIMS 1 Secondary Ion Mass Spectrometer Start-Up Project Overview Secondary Ion Mass Spectrometry Overview MiniSIMS:

Untargeted Metabolomics: Tandem LC-MSMS. Column and Flow Rate Selection Insert Barnes table for flow rates and sensitivity gain. Reverse Phase and Normal.

MS Intro. MS requires gas-phase ions, why? MS uses magnetic and electric fields to control the path of a compound based on mass to charge ratio (m/z)

Mass Analyzers: Quadrupole ion trap?

Polybrominated diphenyl ethers by HRGC/HRMS Coreen Hamilton Brian Fowler Louis Haviland Axys Analytical Services, Ltd.

Finnish Custom Lab., Pekka Ravio

* CORRESPONDING AUTHOR Glucagon Bioanalysis by LC-MS: “Unprecedented Level of Sensitivity (10pg/mL) for a Novel Formulation” Jean-Nicholas Mess 1, Louis-Philippe.

Eliminating Molecular Interferences in ICP-MS for the Simplest Method Development Dr. Torsten Lindemann.

Molecular Mass. Mass of Atom Measured in atomic mass unit (amu) 1 amu = 1.66 x g Defined by assigning the mass of 12 amu to the carbon-12 isotope.

Methods for LTQ Orbitrap A Guided Tour with Examples

Secondary Ion Mass Spectrometry A look at SIMS and Surface Analysis.

Mass Spectrometry Quantitative Mass Spectrometry

1 The world leader in serving science DIA: the Why, How, and When…Really…

Melamine and Related Issues Survey There were 19 Survey Respondents and all tested for Melamine The respondents were from many different types of Business.

Chem. 133 – 4/26 Lecture. Announcements Return graded quiz and additional problem Lab – Lab report deadlines (2:4 – Thursday) Today’s Lecture – Mass Spectrometry.

Agenda  Welcome from the Skyline team!  PRM Targeted Proteomics Using Full-Scan MS2  Introduction with Brendan MacLean  PRM Introduced by Bruno Domon.

1 The world leader in serving science I Orbi Tim Stratton Optimizing the Metabolism Experiment:Small Molecule Structure ID and the Orbitrap.

The world leader in serving science For Research Use Only. Not for use in diagnostic procedures Quantitative Analysis of 4 Immunosuppressant Drugs in Whole.

Unstoppable productivity; Uncompromised MS/MS simplicity Thermo Scientific TSQ 8000 Triple Quadrupole GC-MS/MS Unstoppable productivity; Uncompromised.

DIA Method Design, Data Acquisition, and Assessment

Objective  To develop methods for analysis of compounds in organic aerosol particles Why is this important?  Environmental impact  Alternative fuels.

Introduction to Liquid Phase Mass Spectrometry

Quantitation using Pseudo-Isobaric Tags (QuPIT) and Quantitation using Pseudo-isobaric Amino acids in Cell culture (QuPAC) Parimal Samir Andrew J. Link.

Data independent acquisition methods for metabolomics Stephen Tate, Ron Bonner AB SCIEX, 71 Four Valley Drive, Concord, ON, L4K 4V8 Canada A high resolution.

Forensic Toxicology Use Only Analysis of ETG, ETS using the Thermo Scientific Exactive Mass Spectrometer Kent Johnson Fortes lab, Portland Oregon.

Target Analyses in Parallel Reaction Monitoring Mode (PRM)

Metabolomics Part 2 Mass Spectrometry

University of Washington, Department of Genome Sciences

James Byrd, Marta Kozak 28 Apr 2011

DIA: the Why, How, and When…Really…

Chem. 133 – 4/18 Lecture.

MoP-067 Michael Sudakov 1, R.Giles1 and Dimitris Papanastasiou2

Accelerating Research in Life Sciences

Chem. 133 – 4/13 Lecture.

For Forensic Toxicology use Only

N-Linolenoyl-L-glutamine: 1H-NMR (CD3OD) δ: (m, 6H), 4

Agenda Welcome from the Skyline team!

Mass Spectrometry Obaid M. Shaikh.

KTYDSYLGDDYVR Linearity

For Research Use Only. Not for use in diagnostic procedures

Metabolomics Part 2 Mass Spectrometry

High Resolution Quantitation of Microcystins with SCIEX X500R QTOF System KC Hyland.

Now, More Than Ever, Proteomics Needs Better Chromatography

Analytical metrics of yeast proteome analysis using the Q-OT-qIT (Fusion) as compared with qIT-OT (Orbitrap Elite) and Q-OT (Q-Exactive) hybrids. Analytical.

Schematic of the Q-OT-qIT hybrid mass spectrometer (Fusion).

Presentation transcript:

1 The world leader in serving science Proprietary & Confidential WiSIM DIA Method Set-up Orbitrap Fusion MS MS Instrument Control Software v.1.1 SP1

2 WiSIM DIA, how does it work? Unique to the Orbitrap Fusion MS: Three HR/AM SIM are used to cover precursors ions from 400 to 1000 m/z MS1 ultra high resolution quantification In parallel with each HR/AM SIM scan, 17 sequential Ion Trap CID MS/MS with 12 amu isolation windows are acquired to cover the associated 200 amu HR/AM SIM mass range MS2 confirmation SIM amuSIM amuSIM amu tSIM Orbitrap tMS2 Ion Trap

3 WiSIM DIA, how does it work? SIM 400 – 600 m/z MS2 No. 1 … MS2 No.17 1 st MS1 SIM 600 – 800 m/z Ion Trap OT 240k 3.6 sec SIM 800 – 1000 m/z 2 nd MS1 MS2 No. 1 … MS2 No.17 Ion Trap 3 rd MS1 MS2 No. 1 … MS2 No.17 Ion Trap OT 240k OT 240k 17 Windows of 12 amu each 17 Windows of 12 amu each 17 Windows of 12 amu each

4 WiSIM DIA Method Set-up at a glance: SIM , , m/z 200 amu ion trap isolation width AGC: 3e4 50 ms Max injection Profile tMS2 12 amu quadrupole isolation m/z mass range Ion Trap detection AGC: 5e4 35 ms Max Injection CID 30 Rapid Scan rate X3 6 experiments:

5 WiSIM DIA Method Set-up: 6 experiments: -Three HR/AM tSIM -Three Ion trap tMS2 CID

6 Experiment 1: SIM amu SIM amuSIM amu

7 Experiment 2: SIM amuSIM amuSIM amu

8 Experiment 2: SIM amuSIM amuSIM amu

9 Experiment 3: SIM amu SIM amu SIM amu

10 Experiment 4: SIM amuSIM amuSIM amu

11 Experiment 4: SIM amuSIM amuSIM amu

12 Experiment 5: SIM amuSIM amu SIM amu

13 Experiment 6: SIM amuSIM amuSIM amu

14 Experiment 6: SIM amuSIM amuSIM amu

15 WiSIM-DIA advantages Low detection limits, high selectivity is achieved using 240,000 SIM scan. High sensitivity Ion Trap CID MS2 is used for confirmation of the low abundant components. Unsurpassed speed with 2 detectors working in parallel allows for 3.6 s cycle. LOD and LOQ down to 10 attomole. CVs are better than 15 % for 85 % of compounds, better than 20% for 95% of compounds. Linear dynamic range up to 4 orders of magnitude.

16 For More details, please see Application Note 600 And: