Charm Quantitative Milk Aflatoxin Test MRLAFMQ Test

What is Aflatoxin? Aflatoxin is a natural toxin, and potent carcinogen, produced by the mold Aspergillus flavis and Aspergillus parasiticus Aflatoxin is a broader group of mold produced toxins called mycotoxins. Mold growth reduces crop yields and livestock productivity Mold growth can occur pre-harvest and post harvest There are 4 Aflatoxins produced by mold growth in grains: B1, B2, G1, G2. B1 is the predominate form 80%-95% Aflatoxin B1 when ingested by dairy cows is converted to Aflatoxin M1 & M2. M1 is the predominate Aflatoxin found in milk

Corn exposed to moisture for 18 days Properly stored corn

Action Levels of Aflatoxins (MRL) Must Not Exceed Description 0.05 ppb (50 ppt) Milk (Aflatoxin M1)

Introducing the Charm® MRL Aflatoxin Milk Test

Equipment needed to run MRLAFMQ ? ROSA® Reader with 3-line aflatoxin calibration, version 1.09 or higher. (The green label on the bottom of the Rosa Reader indicates it is compatible with the 3 line aflatoxin test.) Or, ROSA Pearl Reader with green label on unit base AFLA Channel, mode 3 Quad or Dual 40oC 15 minute Incubator Charm ROSA 3 Line AFLA Capable Quad 40oC 15-min incubator ROSA Pearl Reader

MRLAFMQ Storage Specifications Store MRLAFMQ Test strips, Aflatoxin M1 Milk Standard refrigerated: 0 to 7°C Remove from container the number of test strips to be used in one day. Keep these test strips at room temperature during daily use for up to 12 hours. Unused test strips should be discarded Use supplied plastic lid to immediately reseal container tightly and return to refrigerated storage

MRLAFMQ Negative Control Use aflatoxin negative raw commingled milk as a Negative Control. Negative Control may be stored up to 72 hours refrigerated (-15°C or below for up to 2 months) Negative Control must be RESULT NEGATIVE with READING = 0 on ROSA Reader

MRLAFMQ Test Procedure

Do not Read “Invalid Strips” where the “C” appears like:

Concentration-Response of MRLAFMQ method Concentration (ppt*) % Positive† on Rosa Reader 0 % 25 50† 100 % 75 *parts per trillion or ng/kg †90% positive with 95% confidence

Calibration Strips for Standard ROSA Readers –3 line for MRLAFMQ Calibrator serial #s match ROSA Reader serial # Read in Sample 3-Line Mode, AFLA Channel Results for NEG & POS Calibration Strips must read within White Labeled Range Do not proceed…” if out of range”

ROSA PEARL Calibration Strips: MRLAFMQ Can be read on any channel For ROSA Pearl Reader only One set for all ROSA tests Can read either side Can ONLY be read under Performance Mode “ESC 5”, 3- Line Mode Calibrator serial #s match Pearl Reader serial # Do not proceed…“if out of range”

Questions? © 2011-2012 Charm Sciences, Inc. All rights reserved. Charm, and ROSA names, are registered trademarks of Charm Sciences, Inc. MRK-604-001 0212