1. N ew S nap TM B eta- L actam

2. Areas neat, clean, orderly, well ventilated, and temperature controlled (4 – 30 0 C or 39.2 – 86 0 F) Adequate lighting – 50 foot-candles at working surface (preferable 100) Incubator/Heater Block with Snap inset è 45±5 0 C è Thermometer, for each incubator è Level W ork A rea

3. P ipettor C alibration Fixed volume pipettors for certified labs – 450ul. Range = 427.5 to 472.5 Calibrated with 10 consecutive weighings once every 6 months. Weighing must be + 5% of the specified volume. Records maintained.

4. P ipettor C alibration Serial number etched or use manufacturers ID number. Tag pipettor with average weight, date, location, and analysts initials. Only the screening labs may use the disposable bulbs supplied by Idexx.

5. T hermometer C alibrations The NIST traceable thermometer calibrated at ice point annually. The in use thermometer calibrated against a NIST traceable at each temperature of use upon receipt and annually. All thermometers must be checked at each temperature of use. Tag includes temperature checked at, date of check, analysts initials and correction factor. Records maintained. 8/25/2005 LT Snap Block # 4P 45.1 - 0.1C

6. R efrigerator T emperatures Thermometers placed – top / bottom shelves. Check daily for Screening / twice a day for Certified Labs. Record adjusted values only. Adjust temperature of the refrigerator if necessary to maintain temperature (0-4.4 0 C). Document. Records maintained.

7. R efrigeration R equirements Remove only enough Snap tests for each use period (i.e., to test one or a small group of incoming loads). Do not store Snap tests at room temperature. Store at 0 to 7 0 C. Do not store food for consumption in the refrigerator. Record date received/analyst and the date opened/analyst on the box of Snap tests.

8. F resh P ositive C ontrols Record the following information from the positive control in the QA/QC record. Idexx Positive Controls must be stored at 0 - 30 0 C. 1. Manufacture. 2. Lot #. 3. Expiration Date. 4. Date & Time made. Re-hydrate with inhibitor free raw milk and let sit for 10 minutes. Good for 24 hrs at room or fridge temps.

9. N egative C ontrols Obtain a raw milk sample from a silo/tanker/holding tank that has been pre-tested and found (NF) with the Snap Beta-Lactam Test and use it as a Negative Control. Identify the negative control sample and include the date, the silo it was taken from, the time and the analyst. Records maintained. Fresh negative control is good for 72h when maintained at 0-4.4 0 C.

10. N ew L ot N umbers Test each new lot with a positive & negative control to make sure they are working properly. Record on separate form. The lot number on the silver package is the 3 digit number followed by two alpha characters. LOT #

11. W ork A rea Wash hands prior to testing samples. Clean counter area and wipe dry. Keep the incubator clean. Wipe off splashed milk. Run Performance Checks each day of testing. Run negative and positive controls for each day of testing. All controls must give appropriate reactions. If out of range, manufacturer should be contacted for corrective action. Records maintained

12. H eater B lock Snap incubator temperature must be checked and results recorded each day of use. 45 + 5 0 C. è The block temperature is checked by placing a standardized thermometer in a tube containing liquid or the bulb submersed in the heating unit. Record time, date, and temperature of the samples as received and tested. Determine sample temperature by inserting a pre-cooled thermometer into the temperature control (TC). Digital thermometers do not need pre-cooling. Do not accept samples that are more than 3/4 full.

13. N ew S nap T est Label each Snap device and each sample tube. Identify the sample being tested (tanker, positive control, negative control). Place the Snap devices on the heater block. Place the test tubes in a rack. (Not in the heater block.) Look for the blue reagent pellet in the bottom of the tube, if not there, tap gently to bring the pellet down. Remove and discard the sample tube cap (s). Leave the pellet in the tube, do not remove the pellet.

14. N ew S nap T est Mix all of the samples and controls by shaking 25 times in 7 seconds through a 1 foot arc. Use within three minutes. All shaking must done vigorously. All Certified Analysts must use a fixed volume pipettor. Use a separate tip for each sample and control. Hold sample at an angle to avoid the foam. The pipettor tip may go into the sample no more than 1 cm.

15. T est P ortion Tilt sample container at a 45 degree angle Pipet up to 1 inch into milk Avoid foam

16. T est P ortion Draw up the sample/control into the pipettor tip and expel to pre-wet the tip. Remove the test portion. Touch off the pipettor tip on a clean dry area inside the sample container.

17. S creening O nly Only screening facilities may use the disposable pipettors provided by IDEXX. Hold the sample at an angle, to avoid foam. With the pipettor provided, place the tip of the pipettor in the sample (no more than 1 cm), draw the test portion up past the indicator line. Remove the pipet from the milk and then touch the pipettor tip to a dry area inside the sample container. Adjust the sample down to the line.

18. N ew S nap T est Carefully add all of the sample to the correctly identified and labeled tube(s). Use a clean pipettor tip/disposable pipettor for each sample and control. The used pipet tips are placed in disinfectant before they are thrown away.

19. N ew S nap T est Agitate all sample/control tube(s) to completely dissolve the blue reagent tablet. Do this by rocking the tubes in a back and forth motion. Incubate the sample/control tube (s) next to the snap device with the corresponding ID. Incubate the tube (s) at 45 + 5 0 C for 5 minutes. A timer must be used. NOTE: a watch, clock… are unacceptable.

20. N ew S nap T est After 5 mins are up, immediately pour the contents of the tube(s) into the proper sample well(s) of the device(s). Make sure the last drop is delivered into the sample well. Once all devices have been snapped, set the timer for 4 minutes. Read immediately.

21. W hen T o S nap Snap the device just as the blue activation circle begins to disappear. Be sure to snap the device down firmly so the top is completely flush with the base. èToo early to snap. èSnap. èToo late to snap. (Washed out)

22. N ew S nap T est The control spot is on the top and the test spot is on the bottom when the activator button is to the right of the sample well. Not Found (NF) - The test spot is darker or equal to the control spot. Positive - The test spot is lighter than the control spot. Interpretation with SnapShot ONLY. NF POS

23. I dexx S napshot R eader Only use the stylus or your fingers when entering information on the Snapshot reader. Make sure the date and time information is correct. Select the test kit type (beta-lactam…). Enter the kit lot number.

24. I dexx S napshot R eader Enter the sample/control identification number. Enter operator ID. Insert the snap device. Push read The daily printout is attached to the daily worksheet.

25. Negative reading = Not Found. Record NF. Positive reading on initial – run new controls and same sample in duplicate by the same analyst. Both duplicates NF with proper control reactions – can receive the load. If one or both of the duplicate tests give a positive result – the load is Presumptive Positive (PP). Fill out positive load form and call the state. S creening T est