Oxygen Requirements 1. Aerobes 2. Anaerobes 3. Facultative 4. Microaerophilic
Agar Stabs & Agar Shakes 1. Liquify 6 NA Deeps in boiling water 2. Boil for 10 minutes to drive out dissolved oxygen 3. Allow 3 NA Deeps to cool and solidify Agar Stabs 4. Temper 3 NA Deeps in water bath at 55 degrees C Agar Shakes
Agar Stabs 1. Single stab with Inoculating needle – straight down, straight back up 1. Micrococcus luteus 2. Staphylococcus epidermidis 3. Clostridium sporogenes Clostridium rubrum Clostridium butrycum
Agar Shakes 1. Inoculate with Inoculating Loop - “shake” test tube so bacteria are equally distributed 1. Micrococcus luteus 2. Staphylococcus epidermidis 3. Clostridium sporogenes Clostridium rubrum Clostridium butrycum
Agar Stab Aerobic
Agar Stab Anaerobic
Agar Stab Facultative
Agar Stab Microaerophilic
Fluid Thioglycollate Broths (FTB) Sodium thioglycollate Binds to and removes free oxygen Resazurin Anaerobic indicator O2 - PINK No O2 - Clear
Oxygen Reqirements
Aerobic Microaerophilic Anaerobic