Intro to Labs 1-4 Labs ‘n stuff

Lab #1 – Diffusion & Osmosis 4 Parts 1. Diffusion – Qualitative 2. Osmosis – Quantitative & Graph 3. Molar Concentration – Quantitative, Graph, and Inference 4. Water Potential – Calculation 4b. Plasmolysis – if you have not scared or scarred me.

Lab #1 – Diffusion & Osmosis Should get good results Simple Understand what you are doing, don’t just follow the steps!! Think of this lab as intro to AP Labs

Lab #1 – Part 1 You will put two substances in a bag made of dialysis tubing. You will determine whether they exit/enter the dialysis tubing bag. To do this, you will use either indicator strips or IKI (potassium Iodide or Lugol’s solution) Data (color) will be recorded in a table

Lab #1 – Part 2 Fill dialysis bags with different concentrations of sucrose solution, place bags in beaker with water. 0.0 M 0.2 M 0.4 M 0.6 M 0.8 M 1.0 M Weigh each (dialysis bag + sucrose solution) BEFORE and AFTER to calculate Percent Change in Mass Make line graph

Lab#1 – Part 2 Why do we use sucrose solution? What would change if we used a salt solution? Which bags will increase in mass? Which bags will decrease in mass? What is responsible for the increase/decrease in mass?

Lab #1 – Part 3 Pieces of potato (or other vegetable) placed in various sucrose solutions What sucrose concentrations we will use? Measure mass of potato before being placed in solutions to calculate Percent Change in Mass Must be left Overnight.

Lab#1 – Part 3 Why do we use sucrose solution? Which potato samples will increase in mass? Which potato samples will decrease in mass? What is responsible for the increase/decrease in mass?

Lab #1 – Part 4 Calculate Water Potential Water potential = Solute potential + Pressure potential Ψ = Ψs + Ψp Ψs = -iCRT i = 1 C = Molar Concentration R = 0.0831 (liter*bar)/(mole*K) T = temperature in Kelvin

What to do next? Organize yourselves into groups of 3-4 Each group is responsible for getting the following: 12 clear or translucent plastic cups (10oz or more) Sharpie Permanent Marker Label Cups Name of group + Period 2 sets of (0.0M, 0.2M, 0.4M, 0.6M 0.8M 1.0M)

When? Friday 12/2 Monday 12/5 Tuesday 12/6 Lab #2 Activity (Toothpickase) Lab #3 Virtual Lab Monday 12/5 Start Lab #1 & Pre-Lab Quiz (12/5) Tuesday 12/6 Finish Lab #1 Start Lab #4 Pre-Lab Quiz – Lab #4 TEST: Labs 1-4 on Monday (12/12)

Lab #2 – Enzyme Kinetics We will do a modified version of Lab #2 Toothpickase Self-Contained Lab Exercise Separate yourself into groups of 3-4 Need: Approximately 600 flat toothpicks Approximately 100 round or reinforced toothpicks Only need to be harder to break than the flat toothpicks

Lab #3 – Mitosis & Crossing Over Virtual Lab Exercise Data Group Assignment (1-8) by teacher. First, identify cells in various stages of Mitosis (including interphase) Second, calculate map units and draw conclusions

Lab #4 - Photosynthesis 2nd Actual Lab 2 Parts Fingers Crossed? Chromatography (Semi-FAIL) Part that fails miserably Fingers Crossed? IF worse comes to worse, you will just analyze given data

Lab #4 – Part 1

Lab #4 – Calculate Rf values

Chromatography http://www.phschool.com/science/biology_place/labb ench/lab4/quiz1.html Use Online Animations: http://www.phschool.com/science/biology_place/labb ench/lab4/concepts2.html

Part that goes well

Part that does not go well

Results Analysis http://www.phschool.com/science/biology_place/labbench/lab4/quiz2.html