Sequencing Using DNA dependant DNA polymerase Initiation & elongation commences Denature & anneal labelled primer * * dCTP dGTP dATP dTTPdCTP dATP dTTPdATP.

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Presentation transcript:

Sequencing Using DNA dependant DNA polymerase Initiation & elongation commences Denature & anneal labelled primer * * dCTP dGTP dATP dTTPdCTP dATP dTTPdATP * * DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase Elongation terminated by dideoxyCTP ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase Elongation continues for remaining molecules ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP dTTPdATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP dTTP dATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase elongation terminated by dideoxyCTP ddCTP Denature & anneal labelled primer * * dCTP dGTP dATP * * ddCTP DNA template ddCTP

Sequencing Using DNA dependant DNA polymerase Multiple products of different length terminated by A dideoxyCTP Theses molecules represent all the positiions within the complementary sequence where a “C” occurs Four separate reactions each terminated by a different dideoxy molecule would represent all bases Ordering these molecules by size allows us to determine the template sequence ddCTP * * * * * * *