Constructing E.coli cells to act like multicellular organisms ——The engineered evolutionary story.

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Presentation transcript:

Constructing E.coli cells to act like multicellular organisms ——The engineered evolutionary story

Approaching to this purpose One-dimension Two-dimension Three-dimension Mathematical modeling

Do E.coli cells have a head?

One-dimension organization The simplest form----two cells What we need are: 1)A protein located at the anterior side of E.coli cells. 2)A self-recognizable domain that act both as a ligand and a receptor.

The anterior located protein Chemoreceptor Complex in Escherichia coli. Tsr CheA CheW

The outer membrane domain can be modified By the monomer of leucine zipper or the IgSF domain. By the hexomer of histidine.

Two-dimension organization What we need are: 1)A protein located in the anterior site that can bind to a shaped mode. 2)A membrane protein that is broadly distributed on the surface of E.coli cells.

OmpC protein An outer membrane protein.

Cell Division Control SulA and MinCD Enrolled in SOS.

Three-dimension: the story of evolution When cell density increases: Expression of modified OmpC via quorum sensing system. (eg. Inserting constructed OmpC sequence after the promoter of LuxR) Randomly bind?

Mathematical modeling What distinguishes multicelluar organisms from unicellular ones. Evolutionary fitness and trade-off.