1. Surface Engineered Bacteria Engineered to Bind and Signal
Fusion Protein
OmpA – C terminal insertion
OmpA-Loop1 insertion
AIDA-1 – N terminal insertion
FecA – loop insertion
Membrane Protein
Add a slide about the concept of the library. 1

2. Selecting/enriching for surface engineered bacteria
Direct Selection
Direct magnetic beads
Indirect selection
MACS
FACS

3. Various Cell Sorting Experiments with his and strep2 -
Indirect Bead Assays
Various Cell Sorting Experiments with his and strep2 -
Assay Results
Direct Bead Assays

4. After Separation
Test: Cell Sorting with AIDA1 + sender constructs (with his and strep2) -
Assay Results
Before Separation
strep2
his

5. Results:
Cell Selection Assays are a Success! Selecting for surface engineered bacteria (such as his and strep2) is possible through either direct or indirect cell separation.