Field Efficacy of VBTTM Application of VBTTM will: Reduce accumulated solid wastes. Reduce power consumption. Reduce undesirable odors. Reduce contaminants in discharged. water (Improve water quality).

Military Application Deployable Aerobic Aqueous Bioreactor Military Application Deployable Aerobic Aqueous Bioreactor* (The “DAAB”) Traditional aeration uses buoyant bubbles buoyant bubbles float to surface contact/DO delivery time is minimal buoyant bubbles are expensive to generate Aerob-A-Jet bubbles are tiny vacuum bubbles are created under a partial vacuum tiny bubbles remain suspended for hours (.25 mm for 10-12 hours) they readily diffuse and disperse optimize aerobic bacterial growth VBA (Vacuum Bubble Aeration) technology replaces large motors 3 hp can replace a 25 hp motor (traditional aeration) VBA is maximized with less mixing Even better results if sludge is not disturbed !! For high strength wastes, VBA has no equal ! traditional aeration can’t handle BOD above 2-3,000 VBA can handle 9,000 BOD! Converts anaerobic to aerobic can dramatically improve aerobic performance in any BOD application Traditional aeration is very common and well established numerous large companies with established world-wide market-share old method of traditional aeration has NSF approval [The competitive landscape Provide an overview of product competitors, their strengths and weaknesses Position each competitor’s product against new product] *Patent Pending

United States Air Force Sam Houston State University

USAF/SHSU The US Air Force and Sam Houston State University are working on a project to develop a self-contained, portable wastewater treatment system using VBTTM to support 1,000 personnel on bases with no pre-existing infrastructure – bare bases.

Average Influent and Effluent Measurements of TSS During Dynamic Operation 160.62 96% Reduction in TSS Concentration (mg/L) 5.62

Average TSS Values Following Conversion to Dynamic Operation Concentration (mg/L)

Average Influent and Effluent Measurements of BOD5 During Dynamic Operation 119.57 92% Reduction in BOD5 Concentration (mg/L) 9.06

Average BOD5 Following Conversion to Dynamic Operation Concentration (mg/L)

Deployable Aerobic Aqueous Bioreactor* - The “DAAB” *Patent Pending

Deployable Aerobic Aqueous Bioreactor* - The “DAAB” A new bioreactor system for treating industrial and municipal wastewaters is easy to deploy and operate. Produces minimal amounts of sludge and odor. Fully functional and meets EPA release requirements in less than 24 hours. *Patent Pending

Deployable Aerobic Aqueous Bioreactor* - The “DAAB” continued Small, flexible foot print. Can be set up as a self contained, stand alone unit or incorporated into an existing 400 sq ft space. Low energy (<10 kW/h daily) requirements. *Patent Pending

Deployable Aerobic Aqueous Bioreactor* - The “DAAB” continued Other uses - sanitation and environmental cleanup for communities affected by natural disasters. *Patent Pending

How DAAB Works Uses bio-augmentation to optimize the growth of healthy biofilms. Optimizes the metabolism of selected wild type microorganisms by increasing their collisions with biodegradable waste products.

How DAAB Works continued The system’s VBTTM provides an oxygen saturated environment to maximize aerobic metabolism of the microorganisms. The DAAB system* utilizes gravity to reduce operational costs. *Patent Pending

What Separates DAAB from Other Portable Wastewater Treatment Units? DAAB generates very little odor; other systems use aerosols to suppress odor. DAAB generates approximately 1% sludge; comparable units generate from 10 - 40% sludge.

What Separates DAAB from Other Portable Wastewater Treatment Units What Separates DAAB from Other Portable Wastewater Treatment Units? continued DAAB produces effluent acceptable to EPA standards within 24 hours following setup. Comparable units require 7 - 30 days to produce an effluent acceptable to EPA standards.

What Separates DAAB from Other Portable Wastewater Treatment Units What Separates DAAB from Other Portable Wastewater Treatment Units? continued DAAB is a fixed film reactor capable of complete digestion, unlike activated sludge systems that do partial digestion and rely on settling to remove residual organic matter.

What Separates DAAB from Other Portable Wastewater Treatment Units What Separates DAAB from Other Portable Wastewater Treatment Units? continued DAAB operates on approximately 1/5 power required to operate units with comparable treatment capacities.

DAAB Flexible Setup

Integrated Technologies BioAmp® VBTTM

Sanitary Wastewater

Before and After Influent Influent Effluent Effluent

Results of Sanitary Wastewater Influent Wastewater* Treated Effluent* BOD5 152.2 mg/L 23.0 mg/L TSS 269.0 mg/L 15.9 mg/L pH 8.3 7.9 Temp 21.7º C 21.5º C *Average Values Eight (8) hours hydraulic retention

Industrial Wastewater

Industrial Wastewater The DAAB system* has undergone extensive testing to establish its ability to degrade numerous VOC’s within 24 hours of exposure. Microorganism degradation of VOC’s is enhanced when provided with the DAAB environment. In the DAAB environment the microorganisms are resistant to VOC concentrations in excess of 80 ppm. *Patent Pending

Industrial Wastewater Volatile Organic Compounds MDL Reporting Limit Influent Average Effluent Average 1,2,4-Trimethylbenzene 0.28 0.94 18.74 < 0.94 1,3,5-Trimethylbenzene 0.26 0.90 3.16 < 0.90 2-Chlorotoluene 0.95 1.184 < 0.95 Chloroethane 0.29 1.00 2.65 < 1.00 Chloroform 0.19 0.64 1.76 1.176 cis-1,-2-Dichloroethene 0.21 0.71 1.478 < 0.71 m,p-Xylene 0.22 0.75 0.8925 < 0.75 Methylene chloride 1.98 5.00 193.264 19.862 Naphthalene 0.24 0.81 135.848 1.026 n-Butylbenzene 0.41 1.39 24.102 1.6 n-Propylbenzene 0.96 1.135 < 0.96 o-Xylene 0.66 1.318 < 0.66 p-Isopropyltoluene 0.35 1.21 2.352 < 1.21 sec-Butylbenzene 0.36 1.23 1.484 < 1.23 Toluene 1.116

Results of Industrial Wastewater Influent Wastewater* Treated Effluent* BOD5 92.2 mg/L 42.2 mg/L TSS 16.0 mg/L 10.6 mg/L pH 7.9 7.4 Temp 24.7º C 25.0º C *Average Values Ten (10) hours hydraulic retention

Summary of Similar Wastewater Technology People Wt. # Capacity Set-Up Hwy Marine Air KW $PG GPD Time in Days Cost of Unit DAAB System YES 550 1000 9,000 29.58 0.003 0.001 10,000 25,000 0.5 $200,000 EEC Global Yes ? 600 41,800 261.0 0.008 31,000 4* $273,000 Hydroxil 500 - 1000 50,000 226.2 0.009 4-5* $250,000 Seapoint No 27,000 208.8 0.01 20,000 2* Smith Loveless 120,000 278.4 0.014 5* $190,000 Orenco 1 4,000 20.88 0.017 1,200 ** $7,800 Transport Daily Power