MICROBIOLOGIC DX. 3 FORMS : ANTIGENS ANTIBODIES SUGESTIVES TESTS

ANTIGENS MICROSCOPIC OBSERVATION salin solution a) wet prep dark field indian ink

Ags. gram stain acid fast stain b) fix prep giemsa hematoxilin argentic stain

Ags.: Bacterian identification. A) cultures: B.A., EMB, SS, LOWENSTEIN B) tissues cultures C) animal inoculations

ags Molecular studies: DNA, probes, Pcr, western-blot, chemioluminiscency

antibodies A) specific tests B) inespecific tests

ANTIBODIES Meanings: has, had, o cross reaction. fever reaction: Widal, Hudlesson and Weil-Felix ASLO: ANTISTREPTOLISINS VDRL: VENERAL DISEASE REACTION LABORARATORY

ABS IG M: TOXOPLASMOSIS (SABIN – FELDMAN) IN NEW BORNE = INFECTION IG G: TOXOPLASMOSIS IS MOTHER’S IG G

INESPECIFIC EXAMPLES WEIL-FELIX VDRL EB TEST

RUTIN TESTS GENERAL TESTS C.B.C. RED CELLS W.B.C. INCREASE: ACUTE Number bacterial INFECTION NORMAL O DECREASE: chronic INFECTION Platelets: decrease gram negative infection.

WBC DIFERENTIAL COUNT NEUTROPHILS INCREASE: ACUTE INFECTION LYNPHOCYTES INCREASE: CHRONIC o VIRAL INFECTION

OTHERS LIKE: CSF o UA. Pleocytosis (leucocytosis spinal fluid) = infection. PMN = ACUTE INF. MN = CHRONIC or VIRAL INF. Leucocyturia = UTI

Clinical case NURSE 30 YEARS OLD. SUPERVISOR ( NIGHT ) “ RAMON GARIBAY “ HOSPITAL, FEVER ( 39 ) HEADACHE DURING 2 WEEKS. Fever reaction (+ )‏ 1:640 (WEIL-FELIX). TX. AMPICILIN

Normal flora 1.- Why flora = most of microorganisms are bacterías How many cells are in the body?:10 14 bacterias : 10

flora Skin, nose,mouth intestinal, urogenital