A SEM image of a cell-seeded PLG scaffold. ( Figure 1 - Scanning electronic photomicrograph of PLGA microspheres obtained by the multiple emulsion W/O/W method. Bar = 5 µm. ( METHODS -Fabrication of polylactide-co-glycolide (PLG) into microspheres prior to forming scaffolds from microspheres. -To achieve proper kinetics, and attenuate protein release, EGF was first incorporated into PLG microspheres used for scaffold fabrication

FIG. 5. Growth factor delivery enhances blood vessel formation. Blood vessels were identified in tissue sections from implanted scaffolds immunostained for mouse CD31 antigen. Experimental conditions not shown (VEGF EGF and VEGF HGF) were similar in appearance to the condition including all three factors. Note that hepatocytes present in the scaffolds are visible as large, round cells. original magnification 400