CRTI RD: Rapid Methods for Emergency Bioassay Chunsheng Li, Health Canada Gary Kramer, Health Canada Baki Sadi, Health Canada Diana Wilkinson, DRDC-Ottawa Kui Yu, National Research Council Yi Cui, National Research Council Edward Lai, Carleton University Amy Hrdina, Carleton University Negar Bahraini, Carleton University

Project Introduction Project Background - Rapid bioassay is required to screen large population - Po-210 and Sr-90 are two high risk but difficult radionuclides Design Objectives - Detection Limit: 10% of derived urine action level (Bq/L) based on 500 mSv committed effective dose equivalent from inhalation - Urine throughput: 120 samples in the first 48 hours per system - Feces throughput: 20 samples in the first 48 hours per system

Project Tasks Developing rapid methods for measuring Po-210 and Sr-90 in bioassay samples using traditional approach - Based on currently available techniques - Focusing on rapidness, sensitivity, accuracy, repeatability, and field deployability etc - Studying the metabolism of Po-210 Exploring the application of new materials and new technology to radiation assessment - Application of quantum dots in radiation measurement - Application of magnetic nano-parrticles in sample preparation

Achievements To Date (I): Rapid Method for Sr-90 Bioassay R Figure 4: This spectrum shows yttrium-90 growth. From top to bottom: no yttrium, yttrium growth after 1 hour, 10 hours, and 96 hours respectively. Spectra from liquid scintillation counter Tri-Carb 3180 TR/SL.

Urine sample Acidification & Transferring (5 minute) Preconditioning & decolourization (10 minutes) Complexation & pH adjustment (5 minutes) Anion exchange separation (15 minutes) Cocktail mixing & Liquid scintillation measurement (15 minutes)

Baki, Sadi, Chunsheng Li, Sara Jodayree, Vera Kochermin, Edward Lai, Gary Kramer A rapid bioassay method for the determination of Sr-90 in human urine samples. Submitted to Health Physics, 2009 Chunsheng Li, Baki Sadi, Gerry Moodie, Joseph daka, Edward Lai, Gary Kramer Field deployable Technique for Sr-90 emergency bioassay. Sumitted to Radiation Protection Dosimetry, 2009 Sample12345 Urine (g) Sr Spiked (Bq) Results from Triathler (Bq)51.2 ± ± ± ± ± 0.3 B ri (%) B r (%)11.1 S B (%)3.2

Achievements To Date (II): Application of QDs Emission Wavelength (nm) CdS CdSe InP HgS CdTe ZnSe InAs HgTe

A Robert Z Stodilka, Jeffrey JL Carson, Kui Yu, Md. Badruz Zaman, Chunsheng Li, and Diana Wilkinson Optical Degradation of CdSe/ZnS Quantum Dots upon Gamma-Ray Irradiation, J. Phys. Chem. C. 2009, 113,

Yu, Kui; Cui, Yi; Zaman, Md. Badruz; Wilkins, Ruth; Li, Chunsheng; Wu, Xiaohua; Ouyang, Jianying; Optical Response of CdSe Quantum Dots to Cs-137 γ Radiation J. Nanoscience and Nanotechnology. 2009, Accepted.

Achievements To Date (III): Application of MNPs in Bioassay

NIP-PMAA-coated magnetic particles NIP-coated magnetic particles Magnetic particles

Achievements To Date (IV): Metabolism of Po-210 in Rats Primary: volatile Po-210 species in excreta - Implicating the accuracy in radiation dose assessment - Implicating in radiation protection - Providing information for bioassay method development Secondary: IR imaging – change in thermal profile for invasive screening Secondary: excretion and bio-kinetics - Excretion via urine - Excretion via feces - Distributions in tissues/organs

THE END