A, RT-PCR analysis of Eker rat leiomyoma and aged matched normal myometrium. A, RT-PCR analysis of Eker rat leiomyoma and aged matched normal myometrium.

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A, RT-PCR analysis of Eker rat leiomyoma and aged matched normal myometrium. A, RT-PCR analysis of Eker rat leiomyoma and aged matched normal myometrium. Agarose gel resolution of products for HMGA2 (280-bp) and HMGA1 (323-bp) amplified from normal myometria (Normal) and uterine leiomyoma (Tumors). Positive control (C) is rat testis; B denotes water blank. GAPDH products (465-bp) are shown as a control for cDNA integrity. B, a quantitative RT-PCR assay using a competitive template was used to assess levels of HMGA2 mRNA in leiomyoma (n = 7) compared with normal myometria (n = 5). HMGA2 levels in 5 μg of total RNA were significantly increased in tumors over normal myometria (Student’s t test, P < 0.01). Parallel quantitative reactions for GAPDH or α-tubulin were used to normalize the HMGA2 product levels to housekeeping gene levels. HMGA2 levels normalized by this method did not vary significantly between tumors and normal tissues; bars, ±SD. Deborah S. Hunter et al. Cancer Res 2002;62:3766-3772 ©2002 by American Association for Cancer Research