At 1 month, control and les rats were fed AL or fasted every other day for IF. Representative images of 1 μm sections, with electron micrograph insets.

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Fig. 3. Expression of Ninj1protein in cortices of the ischemic hemispheres of the rat brain at 4 day post-MCAO. Coronal brain sections were prepared at.
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Fig. 2. Expression of Ninj1 in cortices of ischemic hemispheres of rat brains at 1 day post-MCAO. Coronal brain sections were prepared at 1 day post-MCAO.
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At 1 month, control and les rats were fed AL or fasted every other day for IF. Representative images of 1 μm sections, with electron micrograph insets showing the ventral column of IF or AL control (A, B) and les (C, D). At 1 month, control and les rats were fed AL or fasted every other day for IF. Representative images of 1 μm sections, with electron micrograph insets showing the ventral column of IF or AL control (A, B) and les (C, D). In control spinal cord sections, there appears to be an increase in myelin after IF (A) compared with AL diet (B). Similarly, in les there are more membrane wrappings around axons after the IF diet (D) compared with AL (C). Scale bars: inset, 1 μm; larger image, 1 mm. A quantification of the total proportion of myelinated axons as well as an average g-ratio of myelinated axons from EM images in the table below illustrates that both control and les rats had significantly more myelinated axons and a significantly decreased average g-ratio after IF compared with AL diet. p < 0.05 (Student's t test). n = 4 for each group. Chelsey M. Smith et al. J. Neurosci. 2013;33:8088-8100 ©2013 by Society for Neuroscience