Evaluation of top candidate clones for selective killing in bispecific antibody format. Evaluation of top candidate clones for selective killing in bispecific.

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Evaluation of top candidate clones for selective killing in bispecific antibody format. Evaluation of top candidate clones for selective killing in bispecific antibody format. A, Clones A–D in bispecific antibody (BsAb) format were incubated at the indicated concentrations with T cells and a panel of target cells for 16 hours at an E:T ratio of 5:1. Cytotoxicity was measured by LDH release assay (n = 3 technical replicates for each condition). Error bars, SD. B, Clones A–D in BsAb format were incubated at 0.2 μg/mL with T cells and target cells engineered to express a mini-gene (MG) spanning the AFP158 peptide region for 16 hours at an E:T ratio of 5:1. Cytotoxicity was measured by LDH release assay (n = 3 technical replicates for each condition). Error bars, SD. Hong Liu et al. Clin Cancer Res 2017;23:478-488 ©2017 by American Association for Cancer Research