Adaptive NK cells resist immunosuppression in the tumor microenvironment. Adaptive NK cells resist immunosuppression in the tumor microenvironment. FACS-sorted.

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Adaptive NK cells resist immunosuppression in the tumor microenvironment. Adaptive NK cells resist immunosuppression in the tumor microenvironment. FACS-sorted NK cells were cultured with IL2 (50 IU/mL) and APCs ± Tregs (Treg:NK, 1:2) for 6 days. A, Canonical and (B) adaptive NK cells were separately coincubated with dye-labeled tumor cell lines K562, THP-1, and DU-145 at a 3:1 E:T ratio, and NK-cell cytotoxicity was evaluated over 48 hours by live imaging. Cumulative data (n = 6) of quantified relative killing are shown from two independent experiments as mean ± SEM, and statistical analyses were performed using the mixed-effects model to compare slopes between groups. Dhifaf Sarhan et al. Cancer Immunol Res 2018;6:766-775 ©2018 by American Association for Cancer Research