1. T cells with a ROR1-specific CAR eliminate tumor cells in vivo only with a modified long spacer.
T cells with a ROR1-specific CAR eliminate tumor cells in vivo only with a modified long spacer. Cytotoxicity (A), IFNγ release (B), and proliferation (C) of CD8+ TCM transduced with ROR1-specific R11-CARs with short, intermediate (interm.), long, and long 4/2NQ spacers after coculture with K562 and K562/ROR1 cells. D, antitumor activity of 5 × 106 R11-CAR-T cells with different spacers in NSG mice engrafted with 5 × 105 JeKo-1-ffluc 7 days before T-cell infusion (3 mice per group). Images obtained 1 day before (day 6) and 3 days after (day 10) T-cell injection are shown. E, persistence of ROR1-CAR-T cells in blood 3 days after T-cell transfer. *, statistically significant differences between groups. Data in A to C are representative of experiments with CAR-T cells derived from at least two different donors.
Michael Hudecek et al. Cancer Immunol Res 2015;3:
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