Combination of pulsed-field gel electrophoresis (PFGE) and single-enzyme amplified fragment length polymorphism (SAFLP) for differentiation of multiresistant.

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Combination of pulsed-field gel electrophoresis (PFGE) and single-enzyme amplified fragment length polymorphism (SAFLP) for differentiation of multiresistant Salmonella enterica serotype typhimurium S. Sood, T. Peters, L.R. Ward, E.J. Threlfall Clinical Microbiology and Infection Volume 8, Issue 3, Pages 154-161 (March 2002) DOI: 10.1046/j.1469-0691.2002.00390.x Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 1 (a) PFGE analysis of S. Typhimurium. Lanes 1 and 20: PFGE low-range DNA ladder (New England BioLabs, Hitchin, UK). Lane 2: P5075600. Lane 3: P5075610. Lane 4: P5075620. Lane 5: P5075640. Lane 6: P5075650. Lane 7: P5075660. Lane 8: P5075671. Lane 9: P5075681. Lane 10: P5075691. Lane 11: P5075701. Lane 12: P5075710. Lane 13: P5075720. Lane 14: P5075730. Lane 15: P5075740. Lane 16: P5075750. Lane 17: P50757761. Lane 18: P5075770. Lane 19: P5075780. (b) Dendrogram of the MR S. Typhimurium pulsed-field profiles. From the pulsed-field patterns, genetic similarity was calculated by the Dice coefficient and clustered by an unweighted pair-group arithmetic averaging method. 1, P5075600; 2, P5075610; 3, P5075620; 4, P5075640; 5, P5075650; 6, P5075660; 7, P5075671; 8, P5075681; 9, P5075691; 10, P5075701; 11, P5075710; 12, P5075720; 13, P5075730; 14, P5075740; 15, P5075750; 16, P50757761; 17, P5075770; 18, P5075780. Clinical Microbiology and Infection 2002 8, 154-161DOI: (10.1046/j.1469-0691.2002.00390.x) Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions

Figure 2 SAFLP analysis of MR S. Typhimurium strains using primer HI-C. (a) Lanes 1 and 12: 123-bp molecular weight marker. Lane 2: P5075600. Lane 3: P5075610. Lane 4: P5075620. Lane 5: P5075640. Lane 6: P5075650. Lane 7: P5075660. Lane 8: P5075671. Lane 9: P5075681. Lane 10: P5075691. Lane 11: PCR-negative control. (b) Lanes 1 and 12: 123-bp molecular weight marker. Lane 2: P5075701. Lane 3: P5075710. Lane 4: P5075720. Lane 5: P5075730. Lane 6: P5075740. Lane 7: P5075750. Lane 8: P5075761. Lane 9: P5075770. Lane 10: P5075780. Lane 11: PCR-negative control. Clinical Microbiology and Infection 2002 8, 154-161DOI: (10.1046/j.1469-0691.2002.00390.x) Copyright © 2002 European Society of Clinical Infectious Diseases Terms and Conditions