Cl- channels in basolateral TAL membranes. XVI

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Cl- channels in basolateral TAL membranes. XVI Cl- channels in basolateral TAL membranes. XVI. MTAL and CTAL cells each contain the mRNAs encoding mmClC-Ka and mcClC-Ka Marina V. Mikhailova, Christopher J. Winters, Thomas E. Andreoli Kidney International Volume 61, Issue 3, Pages 1003-1010 (March 2002) DOI: 10.1046/j.1523-1755.2002.00218.x Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 1 A representative beta-actin reverse-transcription-polymerase chain reaction (RT-PCR) control experiment yielding a predicted DNA fragment 400 base pairs in length. Kidney International 2002 61, 1003-1010DOI: (10.1046/j.1523-1755.2002.00218.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 2 Representative reactions using an mRNA template with (RT-PCR) and without (PCR) reverse transcriptase on the primers specific for mmClC-Ka (mm) or mcClC-Ka (mc). Lanes 5 and 6 show a representative PCR reaction using genomic DNA. Kidney International 2002 61, 1003-1010DOI: (10.1046/j.1523-1755.2002.00218.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 3 Conditions for linear PCR product accumulation: The amount of cDNA/sample and the number of cycles. Kidney International 2002 61, 1003-1010DOI: (10.1046/j.1523-1755.2002.00218.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 4 cDNA fragments obtained using mcClC-Ka and mmClC-Ka templates and primers specific either for mcClC-Ka (labeled c) or mmClC-Ka (labeled m). (A) A 1% agarose gel stained with ethidium bromide. (B) Quantitative densitometry tracings in all experiments (N = 5). Kidney International 2002 61, 1003-1010DOI: (10.1046/j.1523-1755.2002.00218.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 5 RT-PCR DNA fragments obtained using poly(A)+mRNA from either inner cortex or outer medulla and primers specific either for mcClC-Ka (labeled c) or mmClC-Ka (labeled m). (A) A 1% agarose gel stained with ethidium bromide. (B) Quantitative densitometry tracings of RT-PCR products in all experiments (N = 5). Kidney International 2002 61, 1003-1010DOI: (10.1046/j.1523-1755.2002.00218.x) Copyright © 2002 International Society of Nephrology Terms and Conditions

Figure 6 RT-PCR DNA fragments obtained using poly(A)+mRNA from either CTAL or MTAL cells and primers specific either for mcClC-Ka (labeled c) or mmClC-Ka (labeled m). (A) A 1% agarose gel stained with ethidium bromide. (B) quantitative densitometry tracings of RT-PRC products in all experiments (N = 5). Kidney International 2002 61, 1003-1010DOI: (10.1046/j.1523-1755.2002.00218.x) Copyright © 2002 International Society of Nephrology Terms and Conditions