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Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden.

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Presentation on theme: "Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden."— Presentation transcript:

1 Analytical Evaluation of Primer Engineered Multiplex Polymerase Chain Reaction– Restriction Fragment Length Polymorphism for Detection of Factor V Leiden and Prothrombin G20210A  Suzanne Huber, Karolyn J. McMaster, Karl V. Voelkerding  The Journal of Molecular Diagnostics  Volume 2, Issue 3, Pages (August 2000) DOI: /S (10) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Photograph of ethidium bromide-stained 3% agarose gel demonstrating primer-engineered PCR analysis for prothrombin G20210A and factor V Leiden. M denotes the molecular weight marker; W denotes water control.Lane 1: Undigested wild-type 506-bp (prothrombin) and 241-bp (factor V) amplicons.Lane 2: A prothrombin G20210/factor V wild type with digested products of 407, 241, and 99 bp. Lane 3: A prothrombin G20210 wild-type/factor V Leiden heterozygote with digestion products of 407, 241, 209, and 99 bp (32-bp product not shown). Lane 4: A prothrombin G20210 wild-type/factor V Leiden homozygote with digestion products of 407, 209, and 99 bp (32-bp product not shown). Lane 5: A prothrombin G20210A heterozygote/factor V Leiden heterozygote with digestion products of 407, 384, 241, 209, and 99 bp (32- and 23-bp products are not shown). The Journal of Molecular Diagnostics 2000 2, DOI: ( /S (10) ) Copyright © 2000 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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