Vav proteins are required for cytoplasmic `nuclei exclusion zones' in mature enterocytes. Vav proteins are required for cytoplasmic `nuclei exclusion zones'

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Vav proteins are required for cytoplasmic `nuclei exclusion zones' in mature enterocytes. Vav proteins are required for cytoplasmic `nuclei exclusion zones' in mature enterocytes. (A) Supranuclear cytoplasm lengths (distance from the top of the nucleus to the cell apex) were plotted for upper zone of cecal enterocytes (n=3 per genotype). A measurement of zero (y-axis) indicates the apex of a nucleus was located at the cell surface. The red bar denotes the average distance per mouse. The intensity of each X correlates with the number of cells of a given measurement (e.g. darker intensity indicates more cells; n=50 cells measured per zone per mouse). (B) The range of nuclear position in all three zones for WT and Vav1/2/3null mice depicted in a cartoon. The average cell heights (from Fig. 3D) are indicated to the right of each cell. The mean nuclear position (± s.e.m.) is in dark blue. The upper and lower limits of nuclear position are in light blue (abutting the dotted red lines). The apical nuclei free area is hashed. The basal nuclei free area is dotted. The asterisk indicates that both the supra- and subnuclear areas of cytoplasm are statistically significantly different in the upper zone enterocytes of Vav1/2/3null compared with WT mice (P<0.001 determined by Student's t-test). John Y. Liu et al. J Cell Sci 2009;122:324-334 © The Company of Biologists Limited 2009