MiRNA expression profiling in adipocytes from wild-type (WT) and leptin-deficient ob/ob mice. miRNA expression profiling in adipocytes from wild-type (WT)

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a b c Additional File 6 qPCR validation of miRNA expression profiles

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miRNA expression profiling in adipocytes from wild-type (WT) and leptin-deficient ob/ob mice. miRNA expression profiling in adipocytes from wild-type (WT) and leptin-deficient ob/ob mice. A: Intensity scatter plot showing comparison of miRNA profiles in adipocytes from WT and ob/ob mice. The 12 miRNAs that are the focus of our detailed analyses are highlighted in red and indicated by a label. B: Validation of miRNA array results for 12 regulated miRNAs by RT-PCR assays. Expression of all miRNAs are normalized to internal control and plotted as fold-change in a log2 scale. □, Array result (n = 2); ■, RT-PCR result (n = 2). Data are expressed as mean ± SE. Huangming Xie et al. Diabetes 2009;58:1050-1057 ©2009 by American Diabetes Association