Schematic representation of a transcriptomic evaluation approach.

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Schematic representation of a transcriptomic evaluation approach. Schematic representation of a transcriptomic evaluation approach. Transcriptomic approaches such as RNA-Seq involve the sampling of specific tissues (e.g. fish gill) of interest (1), extraction of RNA from the tissue (2), construction of cDNA libraries (3) and use of high-throughput sequencing technology that produce short fragmented reads (4). These fragments are then aligned and assembled based on homology to the reference genome of a closely related species or through a de novo assembly of a reference transcriptome (5). Differential expression of transcripts between samples of interest can then be computed and functionally analyzed towards describing gene interactions and potential consequences (6). Richard E. Connon et al. J Exp Biol 2018;221:jeb148833 © 2018. Published by The Company of Biologists Ltd