Hematopoietic potential of HEP from patient-specific iPSC may be reduced. Hematopoietic potential of HEP from patient-specific iPSC may be reduced. (A)

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Hematopoietic potential of HEP from patient-specific iPSC may be reduced. Hematopoietic potential of HEP from patient-specific iPSC may be reduced. (A) Experimental scheme. HEP (VE-Cad+CD73−CD43−CD235a− cells) were isolated by FACS on day 9 and cultured for additional 5 days on OP-9 mouse bone marrow stromal cells with addition of cytokines for 5 days, at which time hematopoietic potential was assessed by measuring CD43+ and CD45+ cells by flow cytometry. (B) Hematopoietic potential (%CD43+ and/or CD45+ cells) of HEP after 5 days of OP-9 coculture is shown. Minimum of 2 independent experiments were performed except for 3 Int5F clones that were differentiated in 1 experiment (control, n = 8; ZF, n = 6; Int5F, n = 3; P = nonsignificant (NS), unpaired Student t test was performed between control and ZF or control and Int5F). NS P values are not shown. Moonjung Jung et al. Blood Adv 2018;2:3553-3565 ©2018 by American Society of Hematology