Stephan Huber, Gerald Braun, Bernd Schröppel, Michael Horster

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Chloride channels ClC-2 and ICln mRNA expression differs in renal epithelial ontogeny Stephan Huber, Gerald Braun, Bernd Schröppel, Michael Horster Kidney International Volume 54, Pages S149-S151 (September 1998) DOI: 10.1046/j.1523-1755.1998.06730.x Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 1 Scheme of growing ureteric bud and cortical collecting duct epithelium in vitro. Monolayer cultures were obtained by microdissecting and explanting the outermost dichotomous branches of outgrowing ureteric trees at various embryonic and postnatal developmental stages. Kidney International 1998 54, S149-S151DOI: (10.1046/j.1523-1755.1998.06730.x) Copyright © 1998 International Society of Nephrology Terms and Conditions

Figure 2 Semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR). (A) ClC-2— (upper gel), ICln- (middle gel), and β-actin— (lower gel) specific polymerase chain reaction (PCR) products amplified from ureteric bud and cortical collecting duct epithelium at various embryonic (E15 to E17) and postnatal (P1 to P14) developmental stages. PCR products were separated by acrylamide gelelectrophoresis, stained by VistraGreen fluorescence dye, and visualized on a Fluorophosphorimager. (B) Amount of DNA in gel bands (A), normalized by the β-actin bands, and plotted against the developmental stages (means ± se, N = 3 to 4). Kidney International 1998 54, S149-S151DOI: (10.1046/j.1523-1755.1998.06730.x) Copyright © 1998 International Society of Nephrology Terms and Conditions