Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active.

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Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. Effect of expression of constitutively active CAMKK2 on AMPK activation and the cell cycle in G361 cells. A, cells were left untransfected, or were transfected with DNAs encoding GFP fused to full-length (1-588, GFP-WT), or truncated (1-471, GFP-CA) CAMKK2. After 36 hours, AMPK activity was measured in immunoprecipitates; significant differences by one-way ANOVA compared with untransfected control (***, P < 0.001) or GFP-WT (††, P < 0.01) are shown. B, analysis by Western blotting of extracts of untransfected cells and cells transfected with DNAs encoding GFP-WT or GFP-CA. Extracts were made after 36 hours and antibodies used were anti-GFP (top), anti-pT172, anti-AMPK-α, or anti-actin. Results show samples from duplicate cell incubations: similar results were obtained in three independent experiments. C, AMPK activity in immunoprecipitates from cells 36 hours after transfection with DNAs encoding GFP-WT or GFP-CA or untransfected controls. Cells were then incubated with or without 10 μmol/L A23187 for 1 hour; results are mean ± SD (n = 2); significant effects by two-way ANOVA compared with vehicle controls; **, P < 0.01, or untransfected controls (††††, P < 0.0001; ††, P < 0.01) are shown. D, analysis by Western blotting of extracts of the same cells as shown in C. E, percentage of cells in G1, S, or G2–M for cells transfected with DNAs encoding GFP, GFP-WT, or GFP-CA (mean ± SD, n = 3). After 36 hours, cells were treated with nocodazole (70 ng/mL) and 18 hours later were fixed, stained with propidium iodide, and the DNA content analyzed by flow cytometry. The flow cytometer was set-up to analyze only cells expressing GFP. Significant differences between the percentage of that cell-cycle phase compared with GFP alone (****, P < 0.0001) or between GFP-WT and GFP-CA (††††, P < 0.0001) are shown (two-way ANOVA). Similar results were obtained in three identical experiments. F, as in E, except that cells were transfected with DNAs encoding GFP, GFP-CA, or GFP-LKB1 plus STRADA and CAB39. Similar results were obtained in two identical experiments. Sarah Fogarty et al. Mol Cancer Res 2016;14:683-695 ©2016 by American Association for Cancer Research