Protein expression profiling of small cell lung carcinoma treated with valproic acid (VPA) and VAC (mafosfamide, doxorubicin and vindesine). Protein expression.

Slides:

Advertisements

Similar presentations

CENTRE FOR BIOTECHNOLOGY

Advertisements

C B *** Cal51 p21 BAX p53 actin PUMA Nutlin3a ABT wt A *** Le Pen et al., figure S mock ABT-737 Nutlin3a ABT-737.

Date of download: 6/24/2016 Copyright © The American College of Cardiology. All rights reserved. From: Inhibition of ErbB2 causes mitochondrial dysfunction.

Fig. 5. Levels of apoptosis-associated proteins following treatment with parthenolide (PT) and balsalazide. HCT116 cell lysates were prepared after treatment.

The Combined Effects of Hematoporphyrin Monomethyl Ether-SDT and Doxorubicin on the Proliferation of QBC939 Cell Lines Lei Liang, Sheng Xie, Lin Jiang,

Crude extracts of Solanum lyratum protect endothelial cells against oxidized low-density lipoprotein-induced injury by direct antioxidant action Wei-Wen.

Amanda M. Nelson, Kathryn L. Gilliland, Zhaoyuan Cong, Diane M

A novel TNFR1-triggered apoptosis pathway mediated by class IA PI3Ks in neutrophils by Barbara Geering, Ursina Gurzeler, Elena Federzoni, Thomas Kaufmann,

Histone deacetylase inhibitors suppress mechanical stress-induced expression of RUNX-2 and ADAMTS-5 through the inhibition of the MAPK signaling pathway.

M. -H. Moon, J. -K. Jeong, Y. -J. Lee, J. -W. Seol, C. J. Jackson, S

by Kumudha Balakrishnan, William G. Wierda, Michael J

Droxinostat, a Histone Deacetylase Inhibitor, Induces Apoptosis in Hepatocellular Carcinoma Cell Lines via Activation of the Mitochondrial Pathway and.

A B C Supplementary Figure S1. Trabectedin decreases viability of primary MPM cell cultures. A and B, dose-dependent impact of trabectedin on epithelioid.

Phosphoinositide 3-kinase inhibitors protect mouse kidney cells from cyclosporine- induced cell death E. Sarró, O. Tornavaca, M. Plana, A. Meseguer, E.

RAF Inhibition Overcomes Resistance to TRAIL-Induced Apoptosis in Melanoma Cells Anja Berger, Sandra-Annika Quast, Michael Plötz, Nicholas-Frederik Kuhn,

Sphingosine-1-phosphate inhibits H2O2-induced granulosa cell apoptosis via the PI3K/Akt signaling pathway Tatsuo Nakahara, M.D., Akira Iwase, M.D., Ph.D.,

Effects of Betulinic Acid Alone and in Combination with Irradiation in Human Melanoma Cells Edgar Selzer, Emilio Pimentel, Volker Wacheck, Werner Schlegel,

Acquired mutations in BCL2 family proteins conferring resistance to the BH3 mimetic ABT-199 in lymphoma by Vicente Fresquet, Melissa Rieger, Carlo Carolis,

Histone deacetylase inhibitors suppress interleukin-1β-induced nitric oxide and prostaglandin E2 production in human chondrocytes N. Chabane, M.Sc.,

Cathepsin-B-dependent apoptosis triggered by antithymocyte globulins: a novel mechanism of T-cell depletion by Marie-Cécile Michallet, Frederic Saltel,

IGF-1 vs insulin: Respective roles in modulating sodium transport via the PI-3 kinase/Sgk1 pathway in a cortical collecting duct cell line E. Gonzalez-Rodriguez,

by Jun Yuan, David B. Lovejoy, and Des R. Richardson

Enhancement of depsipeptide-mediated apoptosis of lung or esophageal cancer cells by flavopiridol: Activation of the mitochondria-dependent death-signaling.

The Bax/Bcl-2 Ratio Determines the Susceptibility of Human Melanoma Cells to CD95/Fas-Mediated Apoptosis Monika Raisova, Amir M. Hossini, Jürgen Eberle,

Volume 141, Issue 2, Pages (August 2011)

by Eleanor J. Molloy, Amanda J. O'Neill, Julie J

Supplemental Figure 1 Li Fraumeni (087) 5C tankyrase1 actin Mock

Rescue of misfolded recoded cystic fibrosis transmembrane conductance regulator (CFTR) channels. a) Representative Western blot obtained from HEK293 cells.

Volume 64, Issue 1, Pages (July 2003)

Volume 42, Issue 2, Pages (February 2005)

Synergistic effect of S63845 with lapatinib, trastuzumab, or docetaxel

Synergistic Cytotoxicity of Sorafenib with Busulfan and Nucleoside Analogs in Human FMS-like Tyrosine Kinase 3 Internal Tandem Duplications–Positive Acute.

Histamine Contributes to Tissue Remodeling via Periostin Expression

Maturation of recoded cystic fibrosis transmembrane conductance regulator (CFTR) channels. a) Representative Western blot obtained from HEK293 cells expressing.

NF-κB Inhibition through Proteasome Inhibition or IKKβ Blockade Increases the Susceptibility of Melanoma Cells to Cytostatic Treatment through Distinct.

Anti-Inflammatory Activity of Sertaconazole Nitrate Is Mediated via Activation of a p38– COX-2–PGE2 Pathway Runa Sur, Jeffrey M. Babad, Michelle Garay,

Volume 41, Issue 2, Pages (January 2011)

PMA/THP-1 lipid uptake. PMA/THP-1 lipid uptake. PMA/THP-1 cells were incubated with 10% vol/vol Calogen for 24 h with and without inhibitors and were stained.

Bax Activation and Induction of Apoptosis in Human Keratinocytes by the Protein Kinase C δ Catalytic Domain Leonid A. Sitailo, Shalini S. Tibudan, Mitchell.

Apoptosis Induction by SAHA in Cutaneous T-Cell Lymphoma Cells Is Related to Downregulation of c-FLIP and Enhanced TRAIL Signaling Nadya Al-Yacoub, Lothar.

Death Receptor-Independent Apoptosis in Malignant Melanoma Induced by the Small- Molecule Immune Response Modifier Imiquimod Michael P. Schön, B. Gregor.

Phosphorylation levels of discoidin domain receptor (DDR)2.

Volume 46, Issue 5, Pages (June 2012)

Honglin Li, Hong Zhu, Chi-jie Xu, Junying Yuan Cell

Activation of Dual Apoptotic Pathways in Human Melanocytes and Protection by Survivin Tong Liu, Diana Biddle, Adrianne N. Hanks, Brook Brouha, Hui Yan,

Valproic acid (VPA) synergises with VAC (mafosfamide, doxorubicin and vindesine) to induce apoptosis in small cell lung carcinoma (SCLC) cell lines. Valproic.

Tumor necrosis factor-α and lipopolysaccharide induce apoptotic cell death in bovine glomerular endothelial cells Udo K. Meßmer, Verena A. Briner, Josef.

PUMA, a Novel Proapoptotic Gene, Is Induced by p53

Valproic acid (VPA) prevents small cell lung carcinoma (SCLC) tumour growth in combination with cyclophosphamide, vindesine and doxorubicin. Valproic acid.

Fig. 6. Effect of SAHA and ML on histone acetylation, BAX, and p21CDKN1A expression.PANC-1 and BxPC-3 cells were incubated for 48 hours with 5 µM.

Volume 111, Issue 3, Pages (November 2002)

Jerry E. Chipuk, Ulrich Maurer, Douglas R. Green, Martin Schuler

(A) qRT-PCR-analysis of P450 aromatase mRNA in brains from control and d-Asp-treated frogs. (A) qRT-PCR-analysis of P450 aromatase mRNA in brains from.

CML-HSA treatment activates MAPK family members ERK1/2 and p38 but not JNK. Samples were taken at the indicated times after 100 μg/ml CML-HSA exposure.

Effect of the selective pharmacological inhibitors used.

Aβ-mediated Ras-MAPK signaling and Cyclin D1 expression in B103 cells are dependent on APP expression and can be reversed with MEK or Ras inhibition. Aβ-mediated.

Hyperthermia-induced ATF2 and NFκB phosphorylation is p38-MAPK mediated. Hyperthermia-induced ATF2 and NFκB phosphorylation is p38-MAPK mediated. The effect.

The dynamics of Akt activation in cultured human keratinocytes.

Oligomeric Aβ42 treatment induces expression of Ras and Cyclin D1 and activation of ERK in primary rat cortical neurons. Oligomeric Aβ42 treatment induces.

The effects of the FASN blocker C75 on cell growth and survival (A, E, and F), on expression/phosphorylation of PI3K and MAPK signaling proteins and on.

by Xuefang Cao, Xingming Deng, and W. Stratford May

Dual apoptotic signaling pathways triggered by IMMU-140: Anti–HLA-DR- and SN-38–mediated signals. Dual apoptotic signaling pathways triggered by IMMU-140:

Staurosporine induces changes in MMP

Effect of CDV on human SF7796 xenografts in vivo.

The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. Serum-starved.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

Effect of bevacizumab on the proliferation of A2780 cells.

LY elicits hyperphosphorylation of Akt.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Effect of PD on LPS-induced activation of ERK1 and ERK2 in human monocytes. Effect of PD on LPS-induced activation of ERK1 and ERK2 in human.

Presentation transcript:

Protein expression profiling of small cell lung carcinoma treated with valproic acid (VPA) and VAC (mafosfamide, doxorubicin and vindesine). Protein expression profiling of small cell lung carcinoma treated with valproic acid (VPA) and VAC (mafosfamide, doxorubicin and vindesine). Cell lines were cultivated for 24 h with or without VPA (1 mM) in combination with mafosfamide (10 µM), vindesine (20 nM) and doxorubicin (0.3 µM). After culture, the cytosolic and mitochondrial fractions were analysed by Western blotting with the indicated antibodies. Protein concentrations were normalised according to actin levels. The purity of mitochondrial and cytosolic fractions was assessed by expression of VDAC1. Results are shown for the H146 cell line. Similar results were obtained with H69 and H526 cell lines. As expected for a histone deacetylase inhibitor, VPA induced hyper- acetylation of histone H3 when added alone or in combination with VAC. In the presence of VPA, the proapoptotic protein Bid was cleaved, generating the processed and active t-Bid isoform (lower band), and cytochrome c was released from the mitochondria into the cytoplasm (compare upper and lower panels). Under these conditions, Bax was concentrated into mitochondria, generating a cleaved 18-kDa isoform (lower band), and expression of BclxL was strongly reduced, while there was only slightly reduction of the level of Bcl-2. VPA induced phosphorylation of H2AX (γH2AX) and Erk1/2 independently of VAC treatment. Roland Hubaux et al. ERJ Open Res 2015;1:00028-2015 ©2015 by European Respiratory Society