The role of oxidative stress in the development of pulmonary arteriovenous malformations after cavopulmonary anastomosis Sunil P. Malhotra, MD, V.Mohan.

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Presentation transcript:

The role of oxidative stress in the development of pulmonary arteriovenous malformations after cavopulmonary anastomosis Sunil P. Malhotra, MD, V.Mohan Reddy, MD, Stephan Thelitz, MD, You-Ping He, PhD, D.Michael McMullan, MD*, Frank L. Hanley, MD, R.Kirk Riemer, PhD The Journal of Thoracic and Cardiovascular Surgery Volume 124, Issue 3, Pages 479-485 (September 2002) DOI: 10.1067/mtc.2002.120346 Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 1 CPA as performed in this model. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 2 Pulmonary HIF-1α protein expression after CPA and PAB. Representative results from Western blot analysis are displayed with corresponding densitometry results. Expression increased 4-fold after CPA and was unchanged after PAB. Data are expressed as means ± SD (n = 3 at each time point). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 3 HO-1 protein expression was upregulated 2.5-fold after CPA (P =.002) and unchanged after PAB. Representative results from Western blot analysis are displayed with corresponding densitometry results. Data are expressed as means ± SD (n = 3 at each time point). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 4 Pulmonary GLUT-1 expression was upregulated 2.5-fold after CPA (P =.02) but was unchanged after PAB. Representative results from Western blot analysis are displayed with corresponding densitometry results. Data are expressed as means ± SD (n = 3 at each time point). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 5 Pulmonary CD62 protein expression was upregulated 3- to 4-fold after CPA (P =.002) and unchanged after PAB. Representative results from Western blot analysis are displayed with corresponding densitometry results. Data are expressed as means ± SD (n = 3 at each time point). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 6 Pulmonary VEGF protein expression was upregulated 3- to 4-fold after both CPA (P =.003) and PAB (P =.008). Representative results from Western blot analysis are displayed with corresponding densitometry results. Data are expressed as means ± SD (n = 3 at each time point). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 7 Pulmonary Flt-1 protein expression was increased 2- to 3-fold after CPA (P =.006) and PAB (P =.01). Representative results from Western blot analysis are displayed with corresponding densitometry results. Data are expressed as means ± SD (n = 3 at each time point). The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 8 Photomicrographs of VEGF immunolocalization in lung tissue 5 weeks after the operation. (Original magnification 200×.) In A there is a lack of staining in the pulmonary arteriolar wall of the sham-operated control specimens. Enhanced endothelial staining 2 weeks after CPA is demonstrated in B. Black arrows indicate stained endothelium. The white arrow identifies the airway wall for comparison. In C increased staining is demonstrated in PAB specimens as well. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions

Fig. 9 Immunolocalization of CD62. (Original magnification 200×.) Shown are photomicrographs of control (A), CPA (B), and PAB (C) specimens 5 weeks after the operation. In A arrows indicate the unstained vessel wall. In B upregulation of angiotensin II after CPA is localized to pulmonary arterial endothelium. The white arrow indicates unstained bronchiolar wall. The PAB specimen in C reveals a lack of increased pulmonary endothelial CD62 expression. The Journal of Thoracic and Cardiovascular Surgery 2002 124, 479-485DOI: (10.1067/mtc.2002.120346) Copyright © 2002 American Association for Thoracic Surgery Terms and Conditions