Bioluminescence imaging facilitates detection of tumor growth and metastasis in mouse orthotopic xenograft model. Bioluminescence imaging facilitates detection.

Slides:

Advertisements

Similar presentations

Supplemental Material for Endothelial CXCR7 Regulates Breast Cancer Metastasis Amanda C. Stacer 1 Joseph Fenner 1 Stephen P. Cavnar 2 Annie Xiao 1 Shuang.

Advertisements

Genetic Manipulation of Brown Fat Via Oral Administration of an Engineered Recombinant Adeno-associated Viral Serotype Vector Wei Huang, Travis McMurphy,

Histogenic analysis confirms prostate cancer metastasis to lung.

Volume 145, Issue 1, Pages (July 2013)

Molecular Imaging in Gastrointestinal Endoscopy

In vivo responses of AMLMLL to ATRi.

The STM1787 promoter in Salmonella is rapidly activated in vivo by the tumor microenvironment. The STM1787 promoter in Salmonella is rapidly activated.

Volume 25, Issue 7, Pages (July 2017)

Validation of St6GalNAc2 as a metastasis suppressor gene.

JNK signaling mediates mammary tumor growth and metastasis to lungs

Volume 18, Issue 2, Pages (February 2010)

Orthotopic lung cancer mouse model.

OBP-401-based fluorescence-guided surgery of orthotopic lung cancer.

CCL5 is critical for the activity of CAFs and miR-CAFs.

A B C p= ns 4T1BR5 GFP expression P10 P20 4T1BR5-Fluc-GFP Count

B cells infiltrate mouse and human pancreatic neoplasia and promote growth of KrasG12D-PDEC in vivo. B cells infiltrate mouse and human pancreatic neoplasia.

CLIC1 promotes tumor cell spreading through effects on MLCK

Interaction of aggregates with CAF network structures.

Peripheral CAF model. Peripheral CAF model. A, bright field (top) and fluorescence (bottom) micrographs of aggregates that contain RFP-tagged 344SQ cells.

Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model in vivo. Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model.

In vivo homing of intravenously injected CSNRDARRC peptide to bladder tumor in rats. In vivo homing of intravenously injected CSNRDARRC peptide to bladder.

SDF-1/CXCR4 axis is involved in CD133+ tumor cell metastasis toward a lymphatic metastasis niche. SDF-1/CXCR4 axis is involved in CD133+ tumor cell metastasis.

coTCRcys-transduced T cells control tumor growth in vivo.

Effects of DPM treatment on tumor volume, tumor mass, and pulmonary metastasis at experimental end point. Effects of DPM treatment on tumor volume, tumor.

IL6 mRNA is not detected in metastatic prostate cancer cells.

AXL is not expressed in human prostate tumors.

In vivo efficacy and specificity of OTL78 in orthotopic and subcutaneous tumor models using the AMI image system. In vivo efficacy and specificity of OTL78.

Overexpression of L1 in CRC cells induces NF-κB activation and suppression of p65 expression blocks the capacity to confer liver metastasis. Overexpression.

MiR-200c suppresses tumor growth and metastasis of CRC in vivo by targeting Sox2. MiR-200c suppresses tumor growth and metastasis of CRC in vivo by targeting.

Overexpression of DDB2 reduces invasive abilities in lungs of aggressive breast tumor cells. Overexpression of DDB2 reduces invasive abilities in lungs.

EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo. EMT gene expression patterns of M-Wnt and E-Wnt cells in vitro and in vivo.

Effect of therapy with S247 on the development of liver metastases after 14 and 21 days of tumor growth. Effect of therapy with S247 on the development.

GA reduces the growth of Caki-1 tumor xenografts.

In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. A,

In vivo efficacy of targeted EDVTM in an orthotopic neuroblastoma mouse model with high EGFR protein levels. In vivo efficacy of targeted EDVTM in an orthotopic.

Antitumor effects of celastrol in vitro and in vivo.

Cocultivation of fibroblasts with epithelial cells (EP156T-luc).

Combined loss of MPG and ATM sensitizes pGBM cells to temozolomide (TMZ) in vivo. Combined loss of MPG and ATM sensitizes pGBM cells to temozolomide (TMZ)

Rif/mDia2 signaling in primary tumor formation and spontaneous metastasis. Rif/mDia2 signaling in primary tumor formation and spontaneous metastasis. A,

CLIC1 supports invadopodia formation in vitro and metastasis in vivo.

GM-CSF is required for CA-MSC–induced tumor metastasis.

Effect of the crystal form and solid dispersion preparations of KRN633 on the growth of human tumor xenografts in mice. Effect of the crystal form and.

Number of identical D-loop DNA clones detected in 10 DNA clones derived from liver tumor tissues. Number of identical D-loop DNA clones detected in 10.

CRA inhibits the growth of human tumor xenografts in vivo.

Example inverse FF-OCT images (left column) and corresponding histology images (right column) of ovarian metastases. Example inverse FF-OCT images (left.

Tumor cell clusters with increased tumorigenesis, metastasis, and CD44 expression. Tumor cell clusters with increased tumorigenesis, metastasis, and CD44.

Fig. 6 Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9 in vivo. Antitumor effect on tumor growth and pulmonary metastasis of CSSD-9.

Enrichment and detection of CTCs from orthotopic xenograft models of GBM. A, immunohistologic analysis of coronal sections showing mCherry expressing GBM8.

CD44 depletion blocks tumor cell aggregation and lung metastasis in vivo. CD44 depletion blocks tumor cell aggregation and lung metastasis in vivo. A,

In vivo bioluminescence imaging of primary tumors and tumor metastasis

Effect of CDV on human SF7796 xenografts in vivo.

PK profile of OTL78. PK profile of OTL78. A, Representative time-dependent whole-body fluorescence images over white light images of a mouse bearing 22Rv1.

The effect of a DIO regimen ± EPA+DHA supplementation on tumor growth.

VEGF165 stimulates migration of HMVECs

Effect of silencing β-catenin on the invasion and metastasis of MHCC97 and Hep3B cells under normoxic and hypoxic conditions. Effect of silencing β-catenin.

SPARC is required for spontaneous metastasis

In vivo effects of TTFields on intradermal tumors in mice.

Proliferation of TA3-Ha and TA3-St cells in vitro and in vivo.

Transfection efficiency and PicoGreen cell survival assays.

Detection of E-cadherin fragments in human prostate cancer metastases.

Mutation of the Smad3 linker phosphorylation sites in 4T1 cells reduces putative stem cell population and tumor initiating frequency of cells from the.

W. chinensis extract attenuates tumor growth of 103E (a subline of CWR22Rv1) engrafted in the prostate of nude mice. W. chinensis extract attenuates tumor.

BV6 increases bone metastasis.

AXL-on tumors are heterogeneous for AXL expression and EdU incorporation. AXL-on tumors are heterogeneous for AXL expression and EdU incorporation. A,

In vivo effect of KIN-193 on PTEN-deficient tumors.

Lnc-C/EBPβ negatively regulates immune-suppressive function of MDSCs in vivo. Lnc-C/EBPβ negatively regulates immune-suppressive function of MDSCs in vivo.

SCLC initiated from pulmonary neuroendocrine cells metastasizes without upregulating NFIB. A and B, Mouse models of SCLC. Rb1flox/flox;Trp53flox/flox;p130flox/flox;R26mTmG.

Tumor progression is required prior to metastasis of SCLC in both CMV TKO and CGRP TKO mice. Tumor progression is required prior to metastasis of SCLC.

Knockdown of ROR1 increases the invasive potential of melanoma cells in vitro and in vivo. Knockdown of ROR1 increases the invasive potential of melanoma.

AXL knockout does not prevent dormancy.

Presentation transcript:

Bioluminescence imaging facilitates detection of tumor growth and metastasis in mouse orthotopic xenograft model. Bioluminescence imaging facilitates detection of tumor growth and metastasis in mouse orthotopic xenograft model. A, PC3-M cells transduced with lentiviruses for firefly luciferase and GFP expression showed high transduction efficiency by comparing phase contrast (left) and fluorescence microscopy (right). Scale bar, 50 μm. B, a luciferase assay of cell dilutions detected as few as 19 cells. C, when PC3-M cells transduced with luciferase virus were implanted orthotopically (3 × 104 cells), tumor growth could be followed in vivo by bioluminescence imaging. D, ex vivo bioluminescence imaging of organs and tissues was used to identify metastases in lung lobes, liver, kidneys, diaphragm, and spleen. Alexandra Hockla et al. Mol Cancer Res 2012;10:1555-1566 ©2012 by American Association for Cancer Research