Periostin Is a Novel Factor in Cardiac Remodeling After Experimental and Clinical Unloading of the Failing Heart William E. Stansfield, MD, Nancy M.

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Presentation transcript:

Periostin Is a Novel Factor in Cardiac Remodeling After Experimental and Clinical Unloading of the Failing Heart William E. Stansfield, MD, Nancy M. Andersen, MD, Ru-Hang Tang, PhD, Craig H. Selzman, MD The Annals of Thoracic Surgery Volume 88, Issue 6, Pages 1916-1921 (December 2009) DOI: 10.1016/j.athoracsur.2009.07.038 Copyright © 2009 The Society of Thoracic Surgeons Terms and Conditions

Fig 1 Overview of mouse experiments and timeline for human tissue procurement. (VAD = ventricular assist device.) The Annals of Thoracic Surgery 2009 88, 1916-1921DOI: (10.1016/j.athoracsur.2009.07.038) Copyright © 2009 The Society of Thoracic Surgeons Terms and Conditions

Fig 2 Periostin expression in experimental pressure overload and relief. (A) Mouse hearts were procured at the respective times with analysis for real-time polymerase chain reaction of periostin expression relative to 18S rRNA (n = 5,4, and 4 for sham, band, and deband mice; *band vs sham, p = 0.002; †deband vs band, p = 0.034). (B) Western blot of periostin protein expression is corroborated by (C) densitometry (n = 2, 3, and 3 for sham, band, and deband mice; *band vs sham, p = 0.042; †deband vs band, p = 0.038.) The error bars in (A) and (C) reflect the standard error. (D) Immunohistochemistry is shown for periostin staining. The Annals of Thoracic Surgery 2009 88, 1916-1921DOI: (10.1016/j.athoracsur.2009.07.038) Copyright © 2009 The Society of Thoracic Surgeons Terms and Conditions

Fig 3 Periostin and fibrosis. (A) Mouse ventricles were stained with picrosirius red to detect collagen deposition at each respective time. (B) Immunohistochemical quantification of whole ventricle collagen staining (n = 4, 13, and 9 for sham, band, and deband; *band vs sham, p = 0.0001; †band vs deband, p = 0.0002). (C) Higher power (×20) objective of collagen-stained sections. (D) Immunohistochemical staining for periostin expression. (Scale bar for C and D = 100 microns.) The Annals of Thoracic Surgery 2009 88, 1916-1921DOI: (10.1016/j.athoracsur.2009.07.038) Copyright © 2009 The Society of Thoracic Surgeons Terms and Conditions

Fig 4 Western blot of periostin in matched human tissue samples before and after treatment with ventricular assist device (VAD). Densitometry of protein expression levels relative to glyceraldehyde-3-phosphate dehydrogenase (GAPDH; n = 4 paired samples; *post-VAD vs pre-VAD, p = 0.077). The Annals of Thoracic Surgery 2009 88, 1916-1921DOI: (10.1016/j.athoracsur.2009.07.038) Copyright © 2009 The Society of Thoracic Surgeons Terms and Conditions