Exogenous magnesium chloride-adenosine triphosphate administration during reperfusion reduces the extent of necrosis in previously ischemic skeletal muscle

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Presentation transcript:

Exogenous magnesium chloride-adenosine triphosphate administration during reperfusion reduces the extent of necrosis in previously ischemic skeletal muscle P.G. Hayes, MD, FRCSC, S. Liauw, MSc, A. Smith, A.D. Romaschin, PhD, P.M. Walker, MD, PhD, FRCSC Journal of Vascular Surgery Volume 11, Issue 3, Pages 441-447 (March 1990) DOI: 10.1016/0741-5214(90)90245-6 Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 The mean percent difference in muscle necrosis (defined as the percent necrosis in the treated muscle minus the percent necrosis in the contralateral control muscle) was calculated for both therapeutic perfusions. Perfusion with Krebs-Henseleit buffer alone is represented as the cross hatched bar and with Krebs-Henseleit buffer supplemented with 2 mmol/L MgCl2-ATP as the empty bar. Error bars represent ± 1 standard deviation from the mean. The statistical significance of the measures was evaluated by unpaired Student t test and found to be significant at p < 0.005, n = 6 for each therapeutic modality. Journal of Vascular Surgery 1990 11, 441-447DOI: (10.1016/0741-5214(90)90245-6) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Vascular resistance was calculated for muscles perfused after ischemia with either Krebs-Henseleit buffer alone (□), or with this buffer supplemental with 2 mmol/L MgCl2-ATP (Δ). No statistically significant difference was evident at any of the reperfusion times surveyed. Journal of Vascular Surgery 1990 11, 441-447DOI: (10.1016/0741-5214(90)90245-6) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Oxygen consumption across the gracilis muscles was calculated at 20, 30, and 40 minutes of reperfusion. Muscles perfused with Krebs-Henseleit buffer supplemented with 2 μmol/L MgCl2-ATP (Δ) showed a significant difference from those perfused with Krebs-Henseleit buffer (□) only after 40 minutes of reperfusion (p < 0.05, ANOVA). Journal of Vascular Surgery 1990 11, 441-447DOI: (10.1016/0741-5214(90)90245-6) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 The relationship between energy loss (defined as the preischemic adenylate energy charge potential minus the end ischemic adenylate energy charge potential) and extent of muscle necrosis was examined for muscles perfused with Krebs-Henseleit buffer with (Δ) and without (□) exogenous MgCl2-ATP. Both sets of data readily fit linear relationships (linear regression analysis, r = 0.78 for Krebs-Henseleit buffer alone and r = 0.95 for buffer with2 mmol/L MgCl2-ATP). By analysis of covariance the slopes of the two lines were significantly different p < 0.01, but the intercepts were not p > 0.05. Journal of Vascular Surgery 1990 11, 441-447DOI: (10.1016/0741-5214(90)90245-6) Copyright © 1990 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions