Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines. Increase in beige/brown adipocyte characteristics.

Slides:

Advertisements

Similar presentations

PIK3CA gene amplification in glioblastoma and squamous cell carcinoma (SCC) of the lung. PIK3CA gene amplification in glioblastoma and squamous cell carcinoma.

Advertisements

Neal et al.Supplementary Figure S1

MicroRNA-101 Inhibits Growth, Proliferation and Migration and Induces Apoptosis of Breast Cancer Cells by Targeting Sex-Determining Region Y-Box 2 Cell.

T3 increased J7-TRα1 cell migration and proliferation both in vitro and in vivo. T3 increased J7-TRα1 cell migration and proliferation both in vitro and.

Overexpression of ornithine decarboxylase enhances endothelial proliferation by suppressing endostatin expression by Takahiro Nemoto, Hisae Hori, Masataka.

Supplementary Figure 2 Secondary transplanted brain tumors from PASC1 express human GH. Immuno-fluorescence staining of brain sections from mice implanted.

25(OH)D and 1,25(OH)2D perfusion treatment effect on cancer-related markers: IF stains illustrating expression levels for Ki-67 (A), cyclin D1 (B), ErbB-2.

Dihydromyricetin decreases the sphere formation through downregulation of Sox2 in human osteosarcoma. Dihydromyricetin decreases the sphere formation through.

Inhibition of FGFR signaling and tumor growth in SNU-16 xenograft model by administration of E7090. Inhibition of FGFR signaling and tumor growth in SNU-16.

Volume 18, Issue 2, Pages (February 2010)

Orthotopic lung cancer mouse model.

Collagen-dependent phosphorylation of eIF4E in PDAC cells.

Blockade of EDN3/EDNRB signaling impairs GSC self-renewal, cell migration, and tumorigenic capacity. Blockade of EDN3/EDNRB signaling impairs GSC self-renewal,

Pharmacologic inhibition of eIF5A hypusination sensitizes KRas-driven tumor cells to MEK and KRas inhibition. Pharmacologic inhibition of eIF5A hypusination.

Expression changes of specific epigenetic-controlled tumor-related genes by the prenatal/maternal BSp treatment. qRT-PCR and Western blot analysis were.

eIF5A-PEAK1 signaling regulates KRAS protein expression.

Src acetylation is critical for its tumorogenesis property.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

SIRT1 is downregulated in human gastric cancer (GC).

ONC201 activates the ISR. ONC201 activates the ISR. (A) Western blotting analysis for ATF4, CHOP, ATF3, and TRB3 on lysates from HCT116 cells cultured.

SW fraction induces S phase arrest and apoptosis.

TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor tissues. TFAP2A is highly expressed in nasopharyngeal carcinoma cells and tumor.

TFAP2A knockdown inhibits tumor growth in vivo.

Key functional sites of SPINDLIN1 could be phosphorylated by Aurora-A.

CDCP1 is required for invadopodia formation and ECM degradation by human breast cancer cells. CDCP1 is required for invadopodia formation and ECM degradation.

Subcutaneous tumor growth was significantly increased in Ogt-Tg/+ mice

Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model in vivo. Antitumor activity of HER2-lytic hybrid peptide in tumor xenograft model.

Mapping the Pirh2 and p73 interaction sites.

Overexpression of JMJD2B in clinical bladder cancers.

Quercetin induces arrest in G1 phase of cell cycle in P39 xenografts.

Quercetin induces apoptosis in P39 xenografts.

Matriptase-2 inhibited breast tumor development in vivo.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

Effect of G9a on the expression of GSH synthesis enzymes.

VEGF expression in neoplastic and normal prostate tissue.

A, TSG-6 staining in TRAMP tissue and TRAMP cell lines.

Depletion of UCP1 and Myf5 population reduced xenograft growth.

Transplantable HMLEHRASV12 xenografts show multilocular lipid droplets and express beige/brown adipocyte–specific proteins. Transplantable HMLEHRASV12.

Overexpression of DDB2 reduces invasive abilities in lungs of aggressive breast tumor cells. Overexpression of DDB2 reduces invasive abilities in lungs.

TDP1 and TOP1 protein levels correlate positively with TDP1 and TOP1 mRNA levels in colorectal cancer cell lines. TDP1 and TOP1 protein levels correlate.

Active AR signaling in enzalutamide-resistant xenograft tumors.

SAF-1 expression in clinical breast cancer tissues.

Endothelial cells/microvasucles are increased in prostate cancer versus normal tissues. Endothelial cells/microvasucles are increased in prostate cancer.

In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. In vivo tumorigenicity of MKN-1 cells with sustained suppression of HDAC2. A,

Effect of IL24 on phosphorylation of eIF2α and proliferation in cancer cells. Effect of IL24 on phosphorylation of eIF2α and proliferation in cancer cells.

N-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. n-3 PUFAs promote endometrial cancer cell apoptosis in vitro and in vivo. HEC-1-A.

Expression pattern of TLR-4 and MyD88 in EOC cells.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

Changes in signal transduction pathway induced by gefitinib.

NT157 treatment inhibits LNCaP xenograft growth and delays castration-resistant progression. NT157 treatment inhibits LNCaP xenograft growth and delays.

PD-L1 is expressed in breast cancer.

Reduced klotho expression in pancreatic cancer.

Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors. Met is expressed in Her2-overexpressing cell lines and Her2 (+) breast tumors.

2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. 2D-PAGE.

Effect of CDV on human SF7796 xenografts in vivo.

Osteoactivin is overexpressed in aggressively bone metastatic 4T1 subpopulations versus weakly bone metastatic breast cancer populations. Osteoactivin.

Effect of SPINDLIN1 protein on cancer cell proliferation and invasion.

Effects of UVB on AKT in SKH-1 mouse epidermis.

Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Enhanced expression of Cap43 gene by nickel in breast cancer cell lines. Expression.

Mean body weights (grams) of athymic female mice implanted with MDA-MB-231 breast carcinoma xenografts. Mean body weights (grams) of athymic female mice.

Increased expression of angiogenic factors and inflammatory cytokines in mice exposed to hypoxia. Increased expression of angiogenic factors and inflammatory.

BMX is a driver of castration resistance in vitro and in vivo.

MYC expression is correlated with dasatinib sensitivity in cancer cell lines and in vivo. MYC expression is correlated with dasatinib sensitivity in cancer.

Increased growth factor receptor expression in lungs and tumors of hypoxic mice. Increased growth factor receptor expression in lungs and tumors of hypoxic.

Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated breast cancer cells. Elevated STAT3 phosphorylation and RANTES levels in tamoxifen-treated.

Depletion of murine Dnmt proteins after 5-Aza-CdR treatment.

CD36 expression in tissue adjacent and distal to the tumor.

RGS16 expression is decreased in chondrosarcoma and RGS16 transfection counteracts the effects of miR-181a on VEGF and MMP1 expression. RGS16 expression.

I3C reduces the level of Cdc25A protein in breast cancer cells.

PD-L1 expression is not associated with PTEN expression status in melanomas. PD-L1 expression is not associated with PTEN expression status in melanomas.

Presentation transcript:

Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines. Increase in beige/brown adipocyte characteristics in the xenografts from additional breast cancer cell lines. Breast cancer cells (2 × 106 cells), purchased from ATCC, were propagated in their respective media, mixed with Matrigel, and implanted subcutaneously (posterior, dorsolateral) in nude mice (n = 5 mice per cell line). A–C, hematoxylin/eosin-stained picture of xenograft sections obtained from MDA-MB-468 cells. A representative picture at low (A) and high magnifications (B and C) is shown. D and E, quantitative real-time PCR analysis using human- and mouse-specific primers in xenografts (5 weeks) from MDA-MB-468 cells. Relative expression levels in the xenografts were compared either with the cells before implantation (human-specific primers) or with mouse tissue at the site of implantation (mouse-specific primers). Data are shown as mean ± SD, (n = 4). F, MDA-MB-468 cells (2 × 106) were mixed with Matrigel and subcutaneously implanted in mice. Mice were killed at different time points and tissue lysates from excised tumors were analyzed by Western blot analysis for different beige/brown-specific proteins. (n = 3 mice per time point). Pooled samples from each time point were analyzed by Western blot analysis. G and H, representative bright field picture of xenograft sections obtained from MDA-MB-231 cells, toluidine blue stain. I and J, quantitative real-time PCR analysis of xenografts obtained after 5 week of implantation of MDA-MB-231 cells compared either with the cells before implantation (human-specific primers) or with mouse tissue at the site of implantation (mouse-specific primers). Data are shown as mean ± SD, (n = 4). K, MDA-MB-231 cells (2 × 106) were mixed with Matrigel and subcutaneously implanted in mice. Mice were killed at different time points and tissue lysates from excised tumors were analyzed by Western blot analysis for different beige/brown–specific proteins. (n = 3 mice per time point). Pooled samples from each time point were analyzed by Western blot analysis. Rajan Singh et al. Mol Cancer Res 2016;14:78-92 ©2016 by American Association for Cancer Research