Maximum likelihood (ML) unrooted tree based on the full-length 16S rRNA genes (A) and 31 conserved single-copy genes (B) showing the phylogenetic position.

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Maximum likelihood (ML) unrooted tree based on the full-length 16S rRNA genes (A) and 31 conserved single-copy genes (B) showing the phylogenetic position of the Gsub bacterium and its close relatives. Maximum likelihood (ML) unrooted tree based on the full-length 16S rRNA genes (A) and 31 conserved single-copy genes (B) showing the phylogenetic position of the Gsub bacterium and its close relatives. GenBank accession numbers of the reference sequences are shown in parentheses, and the Gsub bacterium is found to be closely related to the cluster of endosymbionts of the deep-sea clam, with the highest 16S rRNA gene identity of 94.5% to “Candidatus Ruthia magnifica” strain Cm. The black circles indicate the reference genomes involved in the subsequent genomic comparison. Bootstrap values of >70% are shown. The length of the branch in the horizontal dimension indicates the amount of change in the evolutionary lineage. The bars at the bottom of panels A and B represent 0.02 and 0.05 nucleotide substitutions per position. aff., affiliated; str., strain. Ren-Mao Tian et al. mSystems 2017; doi:10.1128/mSystems.00184-16