1. Supplementary Fig. S1. 16S RNA Neighbor-joining (NJ) tree of Brevibacterium metallicus sp. nov. NM2E3 T (in bold) and related species of genus Brevibacterium. Numbers above branches indicate bootstrap support (>50 %). The scale bar presented 0.01 substitution of the nucleotide.

2. Supplementary Fig. S2. Maximum likelihood tree based on 16S rRNA gene sequences showing the phylogenetic relationships of Brevibacterium metallicus sp. nov. NM2E3 T (in bold) and closely related species (-InL = 6334.6199). Bootstrap values greater than 50% are shown at nodes as percentages of 1000 replicates. Scale Bar represents 0.08 changes per nucleotide position. GenBank accession numbers are given in parentheses.

3. Supplementary Fig. S3. Maximun-parsimony (MP) tree based on 16S rRNA gene sequences, showing the phylogenetic relationships between the novel isolated NM2E3 T (Brevibacterium metallicus sp. nov.) (in bold) and related taxa. Bootstrap values are percentages based on 1000 replicates; values >5 0% are shown. One of the trees obtained by maximun-parsimony method used min-miniheuristics algoritm is shown.

4. Supplementary Fig. S4. Scanning Electron Micrograph (SEM) of cells of Brevibacterium metallicus sp. nov. NM2E3 T. Bar (Ten intervals), 3.0 μm.