Volume 22, Issue 9, Pages (September 2015)

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Volume 22, Issue 9, Pages 1270-1279 (September 2015) Previously Uncultured Marine Bacteria Linked to Novel Alkaloid Production Eun Ju Choi, Sang-Jip Nam, Lauren Paul, Deanna Beatty, Christopher A. Kauffman, Paul R. Jensen, William Fenical Chemistry & Biology Volume 22, Issue 9, Pages 1270-1279 (September 2015) DOI: 10.1016/j.chembiol.2015.07.014 Copyright © 2015 Elsevier Ltd Terms and Conditions

Chemistry & Biology 2015 22, 1270-1279DOI: (10. 1016/j. chembiol. 2015 Copyright © 2015 Elsevier Ltd Terms and Conditions

Figure 1 Colony Morphologies of Gram-Negative Strains on Agar Plates and in Liquid Media (A) Colonies isolated from marine sediment on P1 agar plate. (B) Colonies isolated from marine sediment on P4 agar plate. (C1, C2) Colonies of strain CNU-872 on P1 agar plates. (D) Colonies of strain CNU-893 on P1 agar. (E1, E2) Colony morphologies of strain CNX-216T on P1 and Nutrient agar plates, respectively. (F1, F2) Colonies of strain CUA-101 on P8 agar plates. (G1, G2) Colonies of strain CUA-116 on P9 agar plates. (H) Isolated strain CUA-117 on P1 agar. (I) Colonies of strain CUA-158 on P10 agar. (J1, J2) Colonies of strain CUA-171 on P9 agar plates. (K1, K2) Colonies of strain CUA-420 on P4 agar plates. (L) Colonies of strain CUA-466 on P7 agar. (M1, M2) Colonies of strain CUA-621 on P7 agar plates. (N1, N2) Colonies of strain TAA-239 on P5 agar plates. (O1, O2) Cultured Gram-negative bacteria in liquid media. Chemistry & Biology 2015 22, 1270-1279DOI: (10.1016/j.chembiol.2015.07.014) Copyright © 2015 Elsevier Ltd Terms and Conditions

Figure 2 Phylogeny 16S rRNA maximum-likelihood phylogeny for the 20 strains studied here (bold), the most closely related type strains (indicated by T), and the five closest BLAST matches. New and candidate new taxa are indicated. Bootstrap values ≥50% (from 1,000 replicates) are shown at their respective nodes. Chemistry & Biology 2015 22, 1270-1279DOI: (10.1016/j.chembiol.2015.07.014) Copyright © 2015 Elsevier Ltd Terms and Conditions

Figure 3 Alkaloidal Metabolites Chemical structures of secondary metabolites isolated from two strains, M. alkaloidigena (CNX-216T) and C. alkaloidigena (CNU-914T). Chemistry & Biology 2015 22, 1270-1279DOI: (10.1016/j.chembiol.2015.07.014) Copyright © 2015 Elsevier Ltd Terms and Conditions