1. Volume 19, Issue 9, Pages 1116-1125 (September 2012)
Insights into a Divergent Phenazine Biosynthetic Pathway Governed by a Plasmid-Born Esmeraldin Gene Cluster 
Zhe Rui, Min Ye, Shuoguo Wang, Kaori Fujikawa, Bankole Akerele, May Aung, Heinz G. Floss, Wenjun Zhang, Tin-Wein Yu 
Chemistry & Biology 
Volume 19, Issue 9, Pages (September 2012)
DOI: /j.chembiol
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2. Chemistry & Biology 2012 19, 1116-1125DOI: (10. 1016/j. chembiol. 2012
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3. Figure 1 Phenazine Metabolites Isolated from S. antibioticus Tü 2706
(A) Chemical structures of esmeraldins, saphenyl metabolites, PDC, and PCA.
(B) The green esmeraldin pigments are produced by the wild-type strain of S. antibioticus Tü 2706, which is plated on MS agar medium at 28°C for 7 days.
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4. Figure 2 Genetic Organization of the esm Genes
(A) The key regulatory genes are colored in yellow, while the esmA-D group genes are shown in blue, red, orange, and green, respectively. Bold lines represent the overlapping cosmids, 6B10, 11E12, and 3E11, where the BglII restriction sites are indicated.
(B) Southern analysis of the wild-type (left lane) and LSB017 (right lane) genomic DNAs digested with BglII and probed with 3E11.
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5. Figure 3
HPLC Analysis of the Crude Extracts from Various S. antibioticus Tü 2706 Strains
(A) The wild-type strain.
(B) The LSB017 mutant.
(C) The LSB017 mutant carrying 3E11.
(D) The esmA1 mutant.
(E) The esmA2 mutants.
(F–J) The esmB1–B5 mutants.
(K) The esmC mutant.
(L–N) The esmD1–D3 mutants.
The crude extracts from 6 day cultures were injected at 40 μl scale for each analysis. The dashed lines indicate the peaks of related phenazine metabolites. The y axis indicates the relative abundance (absorbance). The HPLC profiles of the esmT1, esmT2, and esmA3–A5 mutants are similar to those of the LSB017 mutant (B), and the profiles of the esmT3–T6, esmH2, and esmA6–A8 mutants highly resemble (C). The asterisk marks esmeraldic acid, which is heavily accumulated upon prolonged culture of the wild-type strain and the LSB017 mutant carrying 3E11.
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6. Figure 4
Heterologous Expressions of PhzA-G, EsmA1, and EsmA2 in E. coli BL21 (DE3) pLysS
Bars represent amounts of PDC, PCA, and phenazine accumulated by E. coli BL21 strains containing the corresponding genes (see Tables S3 and S4). Data are averages from triplet cultures; error bars show SDs.
Chemistry & Biology  , DOI: ( /j.chembiol )
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7. Figure 5 Proposed Pathway of Saphenamycin Biosynthesis
Chemistry & Biology  , DOI: ( /j.chembiol )
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