Life cycle stages of Neospora caninum.

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Life cycle stages of Neospora caninum. Life cycle stages of Neospora caninum. (A) Impression smear of the liver of an experimentally infected mouse depicting numerous tachyzoites (Giemsa stain). Notice that the tachyzoites vary in dimension, depending on the stage of division: (a) a slender tachyzoite, (b) a tachyzoite before division, and (c) three dividing tachyzoites compared with the size of a red blood cell (arrow). (B) Histological section of a tissue cyst inside a neuron in the spinal cord of a congenitally infected calf (hematoxylin and eosin stain). Note the thick cyst wall (opposing arrowheads) enclosing slender bradyzoites (open triangle). The host cell nucleus (arrow) is cut at an angle. (C) Unsporulated oocyst (arrow) with a central undivided mass in the feces of a dog (unstained). Bar, 10 μm. (D) Sporulated oocyst (arrow) with two internal sporocysts (unstained). Bar, 10 μm. J. P. Dubey et al. Clin. Microbiol. Rev. 2007; doi:10.1128/CMR.00031-06