Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. Detection and tracking of individual SNAP-tagged proteins on.

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Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. Detection and tracking of individual SNAP-tagged proteins on the surface of living cells. (A) Enlarged view of single SNAP-CD86 particles on the surface of a living cell visualized by TIRF-M. Particles were automatically detected and tracked. The current position (blue circle) and trajectory (blue spline) of each particle are indicated. Apparent merging and splitting events are shown as green and red segments, respectively. (Scale bar, 5 μm.) (B) Same tracks on inverted image (white background). Inset, higher magnification. Davide Calebiro et al. PNAS 2013;110:2:743-748 ©2013 by National Academy of Sciences