Chondroprotective activity of N-acetyl phenylalanine glucosamine derivative on knee joint structure and inflammation in a murine model of osteoarthritis

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Chondroprotective activity of N-acetyl phenylalanine glucosamine derivative on knee joint structure and inflammation in a murine model of osteoarthritis F. Veronesi, G. Giavaresi, M. Maglio, A. Scotto d'Abusco, L. Politi, R. Scandurra, E. Olivotto, B. Grigolo, R.M. Borzì, M. Fini Osteoarthritis and Cartilage Volume 25, Issue 4, Pages 589-599 (April 2017) DOI: 10.1016/j.joca.2016.10.021 Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Experimental design: schedule of surgery, treatments and euthanasia for DMM, DMM + NAPA and NO DMM groups. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Lateral (LFC) and medial (MFC) femoral condyles, lateral (LTP) and medial (MTP) tibial plateau of right knee joints from DMM, DMM + NAPA and NO DMM groups. Safranin O and Fast Green staining. Magnification: 40×. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 3 Beeswarm boxplots (mean and BCa 95% CI) of normalized histological scores and histomorphometric parameters of the right knee joints of the DMM group compared to the DMM + NAPA group. **P < 0.005; ***P < 0.0005. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 4 Immunostaining of lateral (LFC) and medial (MFC) femoral condyles, lateral (LTP) and medial (MTP) tibial plateau of right knee joints from DMM, DMM + NAPA and NO DMM groups for: a) ADAMTS5 and b) IKKα. Magnification: 20×. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 5 Immunostaining of lateral (LFC) and medial (MFC) femoral condyles, lateral (LTP) and medial (MTP) tibial plateau of right knee joints from DMM, DMM + NAPA and NO DMM groups for: a) MMP10 and b) MMP13. Magnification: 20×. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 6 Beeswarm boxplots (mean and BCa 95% CI) of normalized ADAMTS5, MMP10, MMP13 and IKKα protein levels in the right knee joints of the DMM group compared to the DMM + NAPA group. **P < 0.005; ***P < 0.0005. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Fig. 7 Beeswarm boxplots (mean and BCa 95% CI) of normalized SB microhardness (HV) results of DMM and DMM + NAPA groups. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions

Supplementary Fig. 1 Lateral (LFC) and medial (MFC) femoral condyles, lateral (LTP) and medial (MTP) tibial plateau of right knee joints from all the six cases of DMM + NAPA group. Safranin O and Fast Green staining. 15 Magnification: 40×. Osteoarthritis and Cartilage 2017 25, 589-599DOI: (10.1016/j.joca.2016.10.021) Copyright © 2016 Osteoarthritis Research Society International Terms and Conditions