Gene delivery to in situ veins: Differential effects of adenovirus and adeno-associated viral vectors Mohammad H. Eslami, MD, Sidhu P. Gangadharan, MD,

Slides:

Advertisements

Similar presentations

Endothelial cell seeding reduces thrombogenicity of Dacron grafts in humans Per Örtenwall, MD, PhD *, Hans Wadenvik, MD, PhD **, Jack Kutti, MD, PhD **,

Advertisements

Dermal tissue fibrosis in patients with chronic venous insufficiency is associated with increased transforming growth factor-β1 gene expression and protein.

Mohammad H. Eslami, MDa, Sidhu P

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Heat shock protein 27: Induction by gastroduodenal reflux in vivo and augmentation of human esophageal mucosal cell growth in vitro David Mauchley, MD,

Volume 2, Issue 1, Pages (July 2000)

Elastic fibers reconstructed using adenovirus-mediated expression of tropoelastin and tested in the elastase model of abdominal aortic aneurysm in rats

Down-regulation of HLA-G boosted natural killer cell-mediated cytolysis in JEG-3 cells cultured in vitro Li Li Sun, M.Sc., Yibing Han, Ph.D., Jian Hui.

Adenoviral-Mediated Overexpression of Platelet-Derived Growth Factor-B Corrects Ischemic Impaired Wound Healing Kenneth W. Liechty, Mark Nesbit, Meenhard.

An engineered vascular endothelial growth factor-activating transcription factor induces therapeutic angiogenesis in ApoE knockout mice with hindlimb.

Pressure distention compared with pharmacologic relaxation in vein grafting upregulates matrix metalloproteinase-2 and -9 Ada W.Y. Chung, PhD, Pooja.

Tissue engineering applications to vascular bypass graft development: The use of adipose-derived stem cells Paul DiMuzio, MD, Thomas Tulenko, PhD Journal.

Inhibitory Effects of Calcitonin Gene-Related Peptides on Experimental Vein Graft Disease Xiaoning Zhang, MD, Jian Zhuang, MD, Hongsui Wu, MB, Zhihong.

Local lentiviral short hairpin RNA silencing of CCR2 inhibits vein graft thickening in hypercholesterolemic apolipoprotein E3-Leiden mice Daniël Eefting,

Vascular smooth muscle cell peroxisome proliferator-activated receptor-γ deletion promotes abdominal aortic aneurysms Milton Hamblin, PhD, Lin Chang,

Adenoviral-mediated expression of antisense RNA to basic fibroblast growth factor reduces tangential stress in arterialized vein grafts Abigail K. Hanna,

Volume 16, Issue 3, Pages (March 2008)

Somatostatin gene transfer and expression in endothelial cells

Mark Wengrovitz, MD. , Lulseged G. Selassie, MD. , Robert R. M

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Adenovirus-mediated intra-arterial delivery of cellular repressor of E1A-stimulated genes inhibits neointima formation in rabbits after balloon injury

Thomas E. Arnold, MD, Dmitri Gnatenko, PhD, Wadie F. Bahou, MD

Dennis R. Holmes, MD, William Wester, BA, Robert W

Effect of microRNA-145 to prevent vein graft disease in rabbits by regulation of smooth muscle cell phenotype Motoaki Ohnaka, MD, Akira Marui, MD, PhD,

Volume 3, Issue 5, Pages (May 2001)

Comparison of two methods of in vivo gene transfer by electroporation

Magnetic nanosphere-guided site-specific delivery of vascular endothelial growth factor gene attenuates restenosis in rabbit balloon-injured artery Tiemin.

Vascular endothelial growth factor naked DNA gene transfer enhances thrombus recanalization and resolution Matthew Waltham, MA, PhD, Kevin Burnand, MS,

Mark F. Fillinger, MD, Susan E. O'Connor, MD, Robert J

Antisense to transforming growth factor-β1 messenger RNA reduces vein graft intimal hyperplasia and monocyte chemotactic protein 1 Randal A. Wolff, PhD,

Mechanisms of arterial graft failure. II

Michael A. Golden, MD, Y. P. Tina Au, PhD, Richard D

Malcolm O. Perry, MD, Richard Kempczinski, MD

Vascular permeability effect of adenovirus-mediated vascular endothelial growth factor gene transfer to the rabbit and rat skeletal muscle Lioubov Poliakova,

Volume 2, Issue 1, Pages (July 2000)

In vivo suppression of vein graft disease by nonviral, electroporation-mediated, gene transfer of tissue inhibitor of metalloproteinase-1 linked to the.

Antisense basic fibroblast growth factor gene transfer reduces early intimal thickening in a rabbit femoral artery balloon injury model David G. Neschis,

Volume 9, Issue 4, Pages (April 2004)

Survivin expression is up-regulated in vascular injury and identifies a distinct cellular phenotype Hector F. Simosa, MD, Grace Wang, MD, XinXin Sui,

Adventitial delivery of platelet-derived endothelial cell growth factor gene prevented intimal hyperplasia of vein graft Mitsuteru Handa, MD, Wei Li,

Local treatment with recombinant tissue factor pathway inhibitor reduces the development of intimal hyperplasia in experimental vein grafts Tam T.T.

Adventitial versus intimal liposome-mediated ex vivo transfection of canine saphenous vein grafts with endothelial nitric oxide synthase gene Manju Kalra,

Retroviral-mediated transduction of endothelial cells with the lac Z gene impairs cellular proliferation in vitro and graft endothelialization in vivo

Xiuwu Zhang, Chuan-Yuan Li Molecular Therapy

Retroviral vector–mediated transfer and expression of human tissue plasminogen activator gene in human endothelial and vascular smooth muscle cells Daryoush.

Adenovirus Virion Stability and the Viral Genome: Size Matters

Thrombolysis for experimental deep venous thrombosis maintains valvular competence and vasoreactivity Jeffrey M. Rhodes, MD, Jae-Sung Cho, MD, Peter.

John Blebea, MD, Robert A. Cambria, MD, David DeFouw, PhD, Richard N

Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model

Dermal tissue fibrosis in patients with chronic venous insufficiency is associated with increased transforming growth factor-β1 gene expression and protein.

A new animal model for pulmonary hypertension based on the overexpression of a single gene, angiopoietin-1 Danny Chu, MD, Christopher C Sullivan, MS,

Volume 5, Issue 6, Pages (June 2002)

Robert A. McCready, M. D. , Margaret A. Price, B. S. , Richard J

The vascular endothelial cell as a vehicle for gene therapy

Transarterial wall oxygen gradients at a prosthetic vascular graft to artery anastomosis in the rabbit Steven M. Santilli, MD, PhD, Shane E. Wernsing,

The effect of carbon coating and porosity on early patency of expanded polytetrafluoroethylene grafts: An experimental study Donald L. Akers, MD, Yong.

Boulos Toursarkissian, MD, David Schwartz, MD, PhD, Paul R

Zeljko S. Radic, MD, Martin K. O'Donohoe, MB, FRCSI, Lewis B

Differential effects of a gram-negative and a gram-positive infection on autogenous and prosthetic grafts Kevin J. Geary, MD, Zygmunt M. Tomkiewicz,

Michael L. Marin, MD, Ronald E. Gordon, PhD, Frank J

Tissue inhibitor of metalloproteinase-1 is increased in the saphenofemoral junction of patients with varices in the leg Jose R. Parra, MD, Robert A.

Single-Shot, Multicycle Suicide Gene Therapy by Replication-Competent Retrovirus Vectors Achieves Long-Term Survival Benefit in Experimental Glioma Chien-Kuo.

Sidhu P. Gangadharan, MD, Mohammad H. Eslami, MD, Inanna P

Cells derived from omental fat tissue and used for seeding vascular prostheses are not endothelial in origin Michel J.T. Visser, MD, J.Hajo van Bockel,

The effects of endothelial injury on smooth muscle cell proliferation

Differential transcriptional activation of matrix metalloproteinase-2 and membrane type-1 matrix metalloproteinase by experimental deep venous thrombosis.

Adenoviral-mediated uteroglobin gene transfer inhibits neointimal hyperplasia after balloon injury in the rat carotid artery Robert A. Larson, MD, Mina.

George D. Lilly 1906–1988 Journal of Vascular Surgery

James A. DeWeese, MD Journal of Vascular Surgery

Prevention of stenosis after vascular reconstruction: Pharmacologic control of intimal hyperplasia—A review Alexander W. Clowes, MD, Michael A. Reidy,

Presentation transcript:

Gene delivery to in situ veins: Differential effects of adenovirus and adeno-associated viral vectors Mohammad H. Eslami, MD, Sidhu P. Gangadharan, MD, XinXin Sui, MD, Kurt K. Rhynhart, MD, Richard O. Snyder, PhD, Michael S. Conte, MD Journal of Vascular Surgery Volume 31, Issue 6, Pages 1149-1159 (June 2000) DOI: 10.1067/mva2000.106951 Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 1 Schematic representation of transgene constructs used. Adenovirus (Ad ) constructs were inserted into the E1 region of an E1, E3-deleted adenoviral backbone (36 kB). Adeno-associated virus (AAV ) constructs are inserted between the AAV inverted terminal repeats, replacing the AAV coding sequences (4.7 kB). Journal of Vascular Surgery 2000 31, 1149-1159DOI: (10.1067/mva2000.106951) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 2 Analysis of rabbit jugular veins after exposure to adenovirus (A, B, C) or adeno-associated virus (D, E, F) vectors. Specimens were explanted 2 days (A, B) and 14 days (C) after exposure to adenovirus vectors (1·109/mL; β-galactosidase [A, C ]; green fluorescent protein [B] ). A, Inset is a representative area on cross section, demonstrating localization of blue staining to the endothelial monolayer (magnification, 1000×). B, En-face fluorescent microscopy of an adenovirus-green fluorescent protein infected vein is shown. Representative areas of adeno-associated virus-β-galactosidase (1·109/mL) infected veins are shown on days 2 (D) , 14 (E) , and 39 (F) postexposure. F, Inset (magnification, 400×; L, lumen) is a cross section from a densely stained area, demonstrating positive-staining endothelial and smooth muscle cells at day 39. (Magnifications: A and C, 7.5×; B, 200×; D, 20×; E and F, 49×). Journal of Vascular Surgery 2000 31, 1149-1159DOI: (10.1067/mva2000.106951) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 3 Reverse transcriptase-polymerase chain reaction analysis of the green fluorescent protein transgene in target tissue. A, Representative of adeno-associated virus-infected specimens (day 2 , N = 3; day 7 , N = 1; day 14, N = 2; day 30, N = 3). B, Adenovirus specimens (day 2, N = 3; day 7, N = 2; day 14, N = 2; day 30, N = 2). RNA was isolated from veins on the indicated days after viral exposure. Polymerase chain reaction amplification was performed, by using both reverse transcribed (+) and non-reverse transcribed (– ) templates (paired lanes for each tissue sample). M, Size markers (50 bp); P, green fluorescent protein-containing plasmid control; V, control (uninfected) jugular vein; T, testicle from an experimental animal. Journal of Vascular Surgery 2000 31, 1149-1159DOI: (10.1067/mva2000.106951) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 4 Results of ex vivo THP-1 adhesion assays, expressed as a ratio to sham-infected vein segments on the indicated days after vector exposure. Adhesion to adenovirus-infected veins on postexposure day 2 was significantly increased (P <.05), as compared with adeno-associated virus-treated (AAV ) veins and adenovirus infection at all other time points. POD, Postexposure day. Journal of Vascular Surgery 2000 31, 1149-1159DOI: (10.1067/mva2000.106951) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

Fig. 5 Histologic and immunohistochemical analysis of explanted veins after adenovirus or adeno-associated virus mediated gene transfer. A, B, C, Vein specimen harvested 2 days after adeno-associated virus-green fluorescent protein exposure. A, CD31 stain; B, ICAM-1 stain; and C, RAM-11 stain (all magnifications, 100×). D, An adeno-associated virus-green fluorescent protein exposed vein on day 7, ICAM-1 stain (magnification, 100×; inset magnification, 1000×). E (adeno-associated virus-green fluorescent protein) and F (adenovirus-green fluorescent protein), Representative histologic sections from a 30-day explanted vein, stained only with hematoxylin (magnification, 40×). Journal of Vascular Surgery 2000 31, 1149-1159DOI: (10.1067/mva2000.106951) Copyright © 2000 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions