Protective effect of oestrogen receptor α-PvuII transition against idiopathic male infertility: a case-control study and meta-analysis  Narges Mobasseri, Hossein Nikzad, Mohammad Karimian  Reproductive BioMedicine Online  Volume 38, Issue 4, Pages 588-598 (April 2019) DOI: 10.1016/j.rbmo.2019.01.008 Copyright © 2019 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 ERα gene map, PCR_RFLP and DNA sequencing results: [A] Human ERα map was obtained from NCBI with 22 exons, and PvuII [rs2234693] SNP is shown in intron 4; [B] Restriction enzyme map: PvuII restriction enzyme of 369-bp PCR fragment in 1% agarose gel [lane M=100-bp DNA marker; lane 1=CC genotype; lane 2=CT genotype; lane 3=TT genotype]; [C] partial sequences of ERα at the rs2234693 position. Reproductive BioMedicine Online 2019 38, 588-598DOI: (10.1016/j.rbmo.2019.01.008) Copyright © 2019 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Flow diagram of the research selection process. Reproductive BioMedicine Online 2019 38, 588-598DOI: (10.1016/j.rbmo.2019.01.008) Copyright © 2019 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 The forest plots of all selected studies on the association between ERα-PvuII polymorphism and male infertility risk under the co-dominant model [TT vs. CC], heterozygote co-dominant model [CT vs. CC], dominant model [CT+TT vs. CC], and recessive model [TT vs. CC+CT] in an overall analysis. Reproductive BioMedicine Online 2019 38, 588-598DOI: (10.1016/j.rbmo.2019.01.008) Copyright © 2019 Reproductive Healthcare Ltd. Terms and Conditions

Figure 4 Funnel plot of standard error by log odds ratio for the association of ERα-PvuII polymorphism and male infertility in the overall population. Reproductive BioMedicine Online 2019 38, 588-598DOI: (10.1016/j.rbmo.2019.01.008) Copyright © 2019 Reproductive Healthcare Ltd. Terms and Conditions