Heart rhythm disturbances in the neonatal alloxan-induced diabetic rat Frank Christopher Howarth, Mohamed Shafiullah, Ernest Adeghate, Milos Ljubisavljevic, Michael Jacobson  Pathophysiology  Volume 18, Issue 3, Pages 185-192 (June 2011) DOI: 10.1016/j.pathophys.2010.10.001 Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 1 Glucose tolerance. Animals treated with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age. Animals were fasted overnight and then received intraperitonealy 2g glucose per kg bodyweight. Blood glucose was measured at time zero and then 30, 60, 120 and 180min after glucose injection. Glucose tolerance tests were performed at (a) 2 and (b) 8 months. Data are mean±SEM, n=6–8 rats, *p<0.01, **p<0.05. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 2 Heart rate of male rats injected with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age and transmitters were implanted at 2 months. Electrocardiogram data acquired 5min per hour, 24h per day: (a) continuous heart rate data and mean trends (inset) and (b) mean heart rate at 2, 6 and 8 months of age. Data are mean±SEM, n=6–8 rats. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 3 Heart rate variability of male rats injected with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age were followed and transmitters were implanted at 2 months. Electrocardiogram data were acquired 5min per hour, 24h per day: (a) continuous heart rate variability data and mean trends (inset) and (b) mean heart rate variability at 2, 6 and 8 months of age. Data are mean±SEM, n=6–8 rats. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 4 QT interval of male rats injected with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age and transmitters were implanted at 2 months. Electrocardiogram data were acquired 5min per hour, 24h per day: (a) continuous QT interval data and mean trends (inset) and (b) mean QT interval at 2, 6 and 8 months of age. Data are mean±SEM, n=6–8 rats. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 5 Corrected QT intervals of male rats injected with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age and transmitters implanted at 2 months. Electrocardiogram data were acquired 5min per hour, 24h per day: (a) continuous corrected QT interval data and mean trends (inset) and (b) mean corrected QT interval at 2, 6 and 8 months of age. Data are mean±SEM, n=6–8 rats. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 6 Physical activity of male rats injected with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age and transmitters were implanted at 2 months. Physical activity data were acquired 5min per hour, 24h per day: (a) continuous physical activity data and mean trends (inset) and (b) mean physical activity at 2, 6 and 8 months of age. Data are mean±SEM, n=6–8 rats, *p<0.05. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions

Fig. 7 Body temperature of male rats injected with alloxan (ALX; 200mg/kg bodyweight, ip) at 5 days of age and transmitters implanted at 2 months. Body temperature data were acquired 5min per hour, 24h per day: (a) continuous body temperature data and mean trends (inset) and (b) mean physical activity at 2, 6 and 8 months of age. Data are mean±SEM, n=6–8 rats. Pathophysiology 2011 18, 185-192DOI: (10.1016/j.pathophys.2010.10.001) Copyright © 2010 Elsevier Ireland Ltd Terms and Conditions