Play with a friend or alone! Paulos Menos II Lab Midterm Jeopardy Play with a friend or alone! Paulos Menos II

Some quick things This doesn’t cover everything. The difficulty distribution of the questions is most likely not equal, I’m not perfect… Don’t get to behind in studying for test 3 as well, it may come sooner than you realize

simply answer the question. happy studying (if possible)! Lab 1 Lab 2 Lab 3 Lab 4 Lab 5 Lab 6 Lab 7 Lab 8 Lab 9 10 20 30 40 50

Lab 1 10 pts What does PPE stand for?

10 pts Personal protective equipment.

Lab 1 20 pts How long should the loop be leaved inside the incenerator?

20 pts Until ¾ of the loop glows red hot

Lab 1 30 pts Which of the following is required PPE for MCB3020 lab? Lab coat Goggles Gloves Long sleeved shirts

30 pts Long sleeved shirts

Lab 1 40 pts What BSL is the microbiology lab at UCF?

40 pts BSL-2

Lab 1 50 pts First aid kits are located where?

50 pts In the hallway between the labs.

Lab 2 10 pts True or False. When holding a microscope, you only need to hold it at the arm.

10 pts False. You need to hold the arm and the base.

Lab 2 20 pts ____________ is a specialized staining method that uses an anionic dye to stain background rather than the cell.

20 pts Negative staining

Lab 2 30 pts Nigrosin is an example of what sort of dye?

30 pts Anionic dyes

Lab 2 40 pts Bacteria that exhibit a variety of cell shapes in a sample are known as ____________?

40 pts Pleomorphic

Lab 2 50 pts Tetracocci are to tetrads as Sarcinae are to what?

50 pts Cuboidal packets

Lab 3 10 pts The primary dye in the gram stain is?

10 pts Crystal Violet

Lab 3 20 points What is the mordant used in the Gram stain?

20 points Gram’s Iodine

Lab 3 30 pts Paulos is performing a Gram stain. He uses the primary dye but gets distracted and forgets to complete the rest of the steps. What color would the bacteria appear to be under his microscope?

30 pts Purple

Lab 3 40 pts The Gram stain is a __________ stain.

40 pts Differential

Lab 3 50 pts What is the decolorizer in the Gram stain? dH2O 70% Ethanol H2O 95% Ethanol

50 pts 95% Ethanol

Lab 4 10 PTS Methylene blue will dye cells what color?

10 pts Blue

Lab 4 20 pts True or False. Steaming is required after the secondary dye is used to stain the cell in Acid-Fast stain.

20 pts False.

Lab 4 30 pts What is the decolorizer in the endospore stain?

30 pts Water

Lab 4 40 pts Ziehl-Neelsen uses what dye as it’s primary dye?

40 pts Carbol fuchsin.

Lab 4 50 pts Which of the following is matched correctly for the Schaeffer-Fulton Method? Malachite green and Methylene blue Carbol fuchsin and Safranin Malachite green and Safranin Carbol fuchsin and Methylene blue Crystal violet and Safranin

50 pts Malachite green and Safranin

Lab 5 10 pts The solid, gelatin-like substance used in petri dishes is known as what?

10 pts Agar

Lab 5 20 pts True or False. You need to sterilize your loop when streaking from different zones on the same petri dish.

20 pts True.

Lab 5 30 pts In which of the zones on a streak plate should you find isolation? Zone I Zone II Zone III Zone IIIB

30 pts Zone IIIB.

Lab 5 40 pts What would the dilution factor be? 1 ml 7 ml 9 ml

40 pts 1/10

Lab 5 50 pts C If you needed to isolate bacteria that formed small white colonies on this agar, which CFU should you choose? A B D

50 pts D D

Lab 6 10 pts _______ is characterized with complete clearing of the red blood cells.

10 pts Beta (β) hemolysis

Lab 6 20 pts Cloudiness around the stab line in motility is a (positive, negative) result.

20 pts Positive

Lab 6 30 pts What inhibits the growth of Gram negative organisms in PEA?

30 pts Phenylethyl Alcohol

Lab 6 40 ___________ fermenters produce light purple to pink colonies on EMB agar.

40 pts 2,3-butanediol

Lab 6 50 pts What percentage of agar is added into motility medium?

50 pts 0.4%

Lab 7 10 pts What does TNTC mean?

10 pts Too numerous to count

Lab 7 20 pts What does TFTC mean?

20 pts Too few to count

Lab 7 30 pts What does CFU stand for?

30 pts Colony forming units.

Lab 7 40 pts What is the viable counting range for CFU’s on a plate.

40 pts 30–300

Lab 7 50 pts What is the dilution in the 6 mL tube?

50 pts 20 CFU/mL This question is set up to be a bit more difficult than anything you would see on a lab exam so you guys can get a bit more practice

Lab 8 10 pts A clearing zone around a colony on milk agar is a (positive, negative) result.

10 pts Positive

Lab 8 20 pts In the Phenol Red Broth, ________ tubes can be used to see if gas production occurred.

20 pts Durham

Lab 8 30 pts True or False. Lactose is read at the bottom of the tube in KIA.

30 pts False

Lab 8 40 KIA is ideally read about how long after its been inoculated.

40 pts 18 hours

Lab 8 50 What percentage of glucose is found in KIA?

50 pts 0.1%

Lab 9 10 True or Fasle? A positive result in the gelatin test will have the medium liquefied after sitting 20 minutes in ice.

10 pts True

Lab 9 20 When the Tryptone broth is used, a positive result will turn the top of the tube what color?

20 pts Cerise

Lab 9 30 True or false? You stab the agar in a Phenylalanine Slant.

30 pts False

Lab 9 40 In the Simmon’s Citrate test, a positive result turns the media what color?

40 pts Blue

Lab 9 50 With the Methyl red tests in the MR-VP, a _____________ fermenter will turn the broth to a red color.

50 pts Mixed acid fermenter