Supplemental Figure 3 A B C T-DNA 1 2 RGLG1 2329bp 3 T-DNA 1 2 RGLG2 ...TGATCAAGACGTGTTTAGTTTCTATCC... ...T-DNA/AGACGTGTTTAGTTTCTATCC... RGLG1 ...TAGATGCGATCGTGCAAGCGAGGTTGG... ...T-DNA/CGATCGTGCAAGCGAGGTTGG... RGLG2 C WT mutant 100bp ladder 1 2 3 1 2 3 PCR reaction type RGLG1 RGLG2 Supplemental Figure 3

Supplemental Figure 3. Characterization of T-DNA insertions in genes RGLG1 and RGLG2. (A) Schematic drawing of intron-exon structure of the coding regions. Positions of T-DNA insertion in the mutant alleles used in this work are indicated by blue arrows. PCR reactions as shown in (C) are indicated by red numbers, with PCR primers symbolized by black arrows. (B) Sequences of the transition between genes and T-DNA. Top, WT sequence, bottom, transition from T-DNA to genomic sequence. (C) PCR reactions demonstrate absence of full length transcripts in both cases. Supplemental Figure 3