Target-Enriched Next-Generation Sequencing Reveals Differences between Primary and Secondary Ovarian Tumors in Formalin-Fixed, Paraffin-Embedded Tissue

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Target-Enriched Next-Generation Sequencing Reveals Differences between Primary and Secondary Ovarian Tumors in Formalin-Fixed, Paraffin-Embedded Tissue Stijn Crobach, Dina Ruano, Ronald van Eijk, Gert Jan Fleuren, Ivonne Minderhout, Ronelle Snowdowne, Carli Tops, Tom van Wezel, Hans Morreau The Journal of Molecular Diagnostics Volume 17, Issue 2, Pages 193-200 (March 2015) DOI: 10.1016/j.jmoldx.2014.10.006 Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 1 Mutational profiles of primary endometrioid and mucinous ovarian tumors (black) and ovarian metastases of CRC (gray). Absolute number of mutations is given per gene. Genes with a low mutation rate (<3 for primary ovarian tumors; <2 for ovarian CRC metastases) are not shown, except for APC. CRC, colorectal cancer. The Journal of Molecular Diagnostics 2015 17, 193-200DOI: (10.1016/j.jmoldx.2014.10.006) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 2 Overview of APC mutations, locations are indicated with arrows, in primary ovarian tumors (A) and ovarian metastases of colorectal tumors (B). The MCR (codon 1286 to 1513) is indicated with dotted lines. MCR, mutation cluster region. The Journal of Molecular Diagnostics 2015 17, 193-200DOI: (10.1016/j.jmoldx.2014.10.006) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

Figure 3 Overview of CTNNB1 mutations, locations are indicated with arrows, in primary ovarian tumors (A) and in ovarian metastases of colorectal tumors (B). Exon 3 (codon 29 to 42) of CTNNB1 is indicated with dotted lines. The Journal of Molecular Diagnostics 2015 17, 193-200DOI: (10.1016/j.jmoldx.2014.10.006) Copyright © 2015 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions