Implementation of air quality control in reproductive laboratories in full compliance with the Brazilian Cells and Germinative Tissue Directive Sandro.

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Implementation of air quality control in reproductive laboratories in full compliance with the Brazilian Cells and Germinative Tissue Directive Sandro C. Esteves, Fabiola C. Bento Reproductive BioMedicine Online Volume 26, Issue 1, Pages 9-21 (January 2013) DOI: 10.1016/j.rbmo.2012.10.010 Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 1 Schematic representation of the cleanroom embryology facility and its associated areas, including air flow patterns and filtration units. The air-handling ventilation unit room has a roof-top air-handling unit that draws outside air through coarse and activated carbon prefilters before it enters into the main ventilation unit. A free-standing main ventilation unit pulls prefiltered outside air and the cleanrooms’ return air through coarse filters, past a 16-unit potassium permanganate-impregnated pelletized coconut shell-based activated carbon filters and then through fine dust filters. Lastly, filtered air enters the cleanrooms through high-efficiency particulate air (HEPA) filter diffusers. Floor- and ceiling-level vents in the cleanrooms return air to the main ventilation unit to be remixed with the existing air. Differential positive pressure is maintained between rooms. The embryology laboratory/anteroom is positive to the operating room, which is positive to both the embryo-transfer room and the dressing room/hallways. Reproductive BioMedicine Online 2013 26, 9-21DOI: (10.1016/j.rbmo.2012.10.010) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 2 Locations of airborne particle count monitoring within the reproductive facilities and associated areas. Reproductive BioMedicine Online 2013 26, 9-21DOI: (10.1016/j.rbmo.2012.10.010) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions

Figure 3 Stratified data analysis by triennium periods of intracytoplasmic sperm injection cycles performed at standard IVF facilities (1999–2001) and cleanroom facilities (2002–2010). Values are expressed as means. MII=metaphase II; 2PN=two pronuclei. Reproductive BioMedicine Online 2013 26, 9-21DOI: (10.1016/j.rbmo.2012.10.010) Copyright © 2012 Reproductive Healthcare Ltd. Terms and Conditions