Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2.

Slides:

Advertisements

Similar presentations

Enhanced DNA damage in IDH1 mutant glioma cells.

Advertisements

Rab11-FIP3 silencing upregulates TCR-CD3 cell surface expression in an Lck-dependent manner. Rab11-FIP3 silencing upregulates TCR-CD3 cell surface expression.

Downregulation of SPRY4 expression is associated with FGFR1–FRS2 activation. Downregulation of SPRY4 expression is associated with FGFR1–FRS2 activation.

Interfering with HMGA2 expression blocks transformation in metastatic lung cancer cells. Interfering with HMGA2 expression blocks transformation in metastatic.

Collagen-dependent phosphorylation of eIF4E in PDAC cells.

Effect of Nf1 knockdown on Wnt pathway in MSC80 cells.

AMPK induces VEGF-A production by upregulating ERK signaling.

ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. ONC201 activates the ATF4 pathway through the eIF2α kinases HRI and PKR. (A) Western.

HIPK2 decreases the stability of Notch1-IC through proteasome-dependent degradation. HIPK2 decreases the stability of Notch1-IC through proteasome-dependent.

eIF5A-PEAK1 signaling regulates KRAS protein expression.

Titration of antisera against the amount of extract loaded on Western blots. Titration of antisera against the amount of extract loaded on Western blots.

Knocking down Wnt3 increases the cells' response to trastuzumab and reduces cells' invasiveness. Knocking down Wnt3 increases the cells' response to trastuzumab.

JAK3A572V mutation causes constitutive JAK3 activity and IL-2–independent proliferation of NKTCL cells. JAK3A572V mutation causes constitutive JAK3 activity.

BCL-3 regulates expression of stemness-associated Wnt targets.

eNOS regulated IR-induced NO generation and EGFR signal activation.

Lats2 functions at the G1–S checkpoint in U2OS cells.

co-IP of LEDGF/p75 and MeCP2.

Targeting eIF4E increases ZEB1 protein and mRNA levels and decreases ZEB1-targeting miR-200c and miR-141 microRNAs. Targeting eIF4E increases ZEB1 protein.

TFAP2A interacts with HIF-1α and regulates VEGF expression and cell proliferation. TFAP2A interacts with HIF-1α and regulates VEGF expression and cell.

A, IGFBP-3 knockdown via siRNA using real-time qRT-PCR analysis.

Activating mutation of Ras is associated with CDCP1 expression in NSCLC. A, NSCLC cell lines with Ras + B-Raf mutations and wild-type Ras were examined.

Representative Western blot analyses of S-phase and other proteins from PC3 cell lysates obtained 3 days after oligomer (400 nm)/Lipofectin (15 μg/ml)

FOXO3a phosphorylation and expression.

SiRNA screening identifies genes required for the viability of Pim1-ovexpressing prostate cells. siRNA screening identifies genes required for the viability.

Synergistic effect of CNP and doxorubicin (DOX) on thiol oxidation.

The role of p53 in H2O2-mediated cell death of hOGG1-deficient H1299 lung carcinoma p53 null cells. The role of p53 in H2O2-mediated cell death of hOGG1-deficient.

Effect of LY on phosphorylation of various MAP kinase substrates in HeLa cells in vitro. Effect of LY on phosphorylation of various MAP kinase.

BME treatment increases granzyme B expression in NK 3.3 cells.

AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2. AG1024 reduced the level of tyrosyl-phosphorylated proteins, including Erk2.

DNA damage signaling regulates mutant p53 ubiquitination.

Imatinib mesylate inhibits PDGF-mediated ERK and Akt activation.

Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells. Mcl-1 knockdown promotes cleavage of caspase-3 in nonadherent melanoma cells.

Sensor siRNAs can be used in high-order combinations.

CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection of CXCR4 siRNAs. CXCR4 expression levels of MDA-MB-231 cells at 48 hours posttransfection.

Effects of visfatin on the cell proliferation and phosphorylation of ERK, Akt, and GSK-3β proteins in HCC cells. Effects of visfatin on the cell proliferation.

Western blot analysis of Wnt signaling genes.

MiR-200c is a PI3K–AKT signaling pathway regulator in CRC

Expression of CRC stem cell markers and L1 in CRC cells.

ONC201 activates an ISR, decreases cyclin D1 expression, and causes an accumulation of cells in G1 in TNBC cells sensitive to the compounds' antiproliferative.

Knockdown of PKM2 suppressed TPA-induced skin cell transformation.

Effect of SFN on the protein expression of Nrf2, HO-1, and NQO1 in JB6-shMock and JB6-shNrf2 cells. Effect of SFN on the protein expression of Nrf2, HO-1,

TFAP2A knockdown inhibits cell growth by targeting HIF-1α signaling.

Effect of IL24 on phosphorylation of eIF2α and proliferation in cancer cells. Effect of IL24 on phosphorylation of eIF2α and proliferation in cancer cells.

IGF-IR in human head and neck cancer.

Targeting DCLK1 by miRNA-137.

PKCζ is tyrosine phosphorylated by EGF and contributes to EGF-induced activation of ERK in Mef cells. PKCζ is tyrosine phosphorylated by EGF and contributes.

SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression. SBC-5 miR-335+, but not SBC-5 miR-29a+, exhibited reduced IGF-IR expression.

Effects of HDAC2 inactivation on the invasive potential of human gastric cancer cells. Effects of HDAC2 inactivation on the invasive potential of human.

The effects of HDAC2 knockdown on cell-cycle proteins.

Changes in signal transduction pathway induced by gefitinib.

2D-PAGE gels showing differentially expressed proteins in Panc-1 cells transfected with siNR4A1 and functional classification of the proteins. 2D-PAGE.

The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. The combination of trastuzumab and SU11274 abrogate Akt phosphorylation. Serum-starved.

Effects of UVB on AKT in SKH-1 mouse epidermis.

PVT1 binds to the FOXM1 protein and increases its protein level by enhancing its stability. PVT1 binds to the FOXM1 protein and increases its protein level.

AS1411 alters subcellular distribution of PRMT5 in a time-dependent, dose-dependent, and nucleolin-dependent manner. AS1411 alters subcellular distribution.

EGCG affects growth factor receptor signaling in H2111, H358, and H460 NSCLC cells. EGCG affects growth factor receptor signaling in H2111, H358, and H460.

HOXA11-AS acts as a ceRNA for miR-1297.

P53 restoration in a syngeneic transplant model of Mdm2Tg p53Neo/Neo CreER angiosarcoma. p53 restoration in a syngeneic transplant model of Mdm2Tg p53Neo/Neo.

Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization in 231BR3 cells. Double knockdown of HER2/EGFR abolishes HPSE nucleolar localization.

Posttranslational phosphorylation of p53 by platinum drugs in ovarian tumor cells. Posttranslational phosphorylation of p53 by platinum drugs in ovarian.

PARP1 suppresses the transcription of PD-L1 in cancer cells.

Association of TCTP with Pim-3 in human pancreatic cancer cell lines.

Depletion of murine Dnmt proteins after 5-Aza-CdR treatment.

Effect of SFN on the total activity and protein expression of HDACs in JB6 P+ cells. Effect of SFN on the total activity and protein expression of HDACs.

Effects of NMT siRNAs on MARCKS and c-Src.

Knockdown of NR4A1 induces endoplasmic reticulum stress–mediated apoptosis by increasing ROS production in pancreatic cancer cells. Knockdown of NR4A1.

miR-181a knockdown inhibits VEGF and MMP1 secretion in vitro.

Knockdown of ROR1 increases the invasive potential of melanoma cells in vitro and in vivo. Knockdown of ROR1 increases the invasive potential of melanoma.

TCTP enhances the protein stability of Pim-3 by blocking the ubiquitin–proteasome degradation of Pim-3. TCTP enhances the protein stability of Pim-3 by.

Time course of NMT knockdown.

Presentation transcript:

Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Proline supplementation during P5CS protein knockdown suppressed GCN2 activation. Melanoma cell lines UACC 903, 1205 Lu, and A375M were transfected with siRNA-targeting ALDH18A1 or a scrambled control siRNA. Cells were maintained in 1.25 mmol/L (UACC 903, A375M) or 2.5 mmol/L (1205 Lu) proline media for 48 or 96 hours before collecting protein lysates. Western blotting compared phosphorylated with total protein levels. Protein band intensity was quantified using ImageJ software, normalized to ERK2 loading control, and expressed as a percentage of the scrambled siRNA blot intensity. Gregory R. Kardos et al. Mol Cancer Res 2015;13:1408-1420 ©2015 by American Association for Cancer Research