Overexpression of Muscarinic Receptor 3 Promotes Metastasis and Predicts Poor Prognosis in Non–Small-Cell Lung Cancer Gengpeng Lin, MD, Longhua Sun,

Slides:

Advertisements

Similar presentations

Curative Treatment of Stage I Non-Small-Cell Lung Cancer in Patients With Severe COPD: Stereotactic Radiotherapy Outcomes and Systematic Review David.

Advertisements

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer Ming-Chuan Wang, PhD, Ying-Hua Chang,

Pathobiological Implications of MUC4 in Non–Small-Cell Lung Cancer

A Case of a Patient with Idiopathic Pulmonary Fibrosis with Lung Squamous Cell Carcinoma Treated with Nivolumab Monica Khunger, MD Journal of Thoracic.

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Molecular Therapy - Oncolytics

Brandon Stone, MD, Victor S. Mangona, MD, Matthew D

The Long Non-Coding RNA XIST Interacted with MiR-124 to Modulate Bladder Cancer Growth, Invasion and Migration by Targeting Androgen Receptor (AR) Cell.

Cell Physiol Biochem 2017;44:1867– DOI: /

Elevated FOXC2 Expression Promotes Invasion of HCC Cell Lines and is Associated with Poor Prognosis in Hepatocellular Carcinoma Cell Physiol Biochem 2017;44:99–109.

NDRG1/Cap43/Drg-1 may Predict Tumor Angiogenesis and Poor Outcome in Patients with Lung Cancer Koichi Azuma, MD, PhD, Akihiko Kawahara, PhD, Satoshi.

Regulation of vascular smooth muscle cell expression and function of matrix metalloproteinases is mediated by estrogen and progesterone exposure Oscar.

Integrin αvβ6 Promotes Lung Cancer Proliferation and Metastasis through Upregulation of IL-8–Mediated MAPK/ERK Signaling Pengwei Yan, Huanfeng Zhu, Li.

WT1 Promotes Invasion of NSCLC via Suppression of CDH1

The Chemokine Receptor CXCR4 and c-MET Cooperatively Promote Epithelial- Mesenchymal Transition in Gastric Cancer Cells Yu Cheng, Yongxi Song, Jinglei.

Deregulation of SLIT2-Mediated Cdc42 Activity Is Associated with Esophageal Cancer Metastasis and Poor Prognosis Ruo-Chia Tseng, PhD, Jia-Ming Chang,

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

High Expression of PHGDH Predicts Poor Prognosis in Non–Small Cell Lung Cancer Jinhong Zhu, Jianqun Ma, Xudong Wang, Tianjiao Ma, Shu Zhang, Wei Wang,

Up-Regulation of RFC3 Promotes Triple Negative Breast Cancer Metastasis and is Associated With Poor Prognosis Via EMT Zhen-Yu He, San-Gang Wu, Fang Peng,

SDHB deficiency promotes TGFβ-mediated invasion and metastasis of colorectal cancer through transcriptional repression complex SNAIL1-SMAD3/4 Haiyu Wang,

IFN-γ Induces Gastric Cancer Cell Proliferation and Metastasis Through Upregulation of Integrin β3-Mediated NF-κB Signaling Yuan-Hua Xu, Zheng-Li Li,

MicroRNA-489 Plays an Anti-Metastatic Role in Human Hepatocellular Carcinoma by Targeting Matrix Metalloproteinase-7 Yixiong Lin, Jianjun Liu, Yuqi Huang,

RET Mutation and Expression in Small-Cell Lung Cancer

Characterization of Aurora A and Its Impact on the Effect of Cisplatin-Based Chemotherapy in Patients with Non–Small Cell Lung Cancer Peng Kuang, Zuhua.

Cell Physiol Biochem 2016;39: DOI: /

Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage- Dominant Microenvironment in Lung Cancer Lunxu Liu, MD, PhD, Dongxia.

Prognostic Significance of TAZ Expression in Resected Non-Small Cell Lung Cancer Mian Xie, MD, PhD, Li Zhang, MD, Chao-Sheng He, MD, Jin-Hui Hou, MD,

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

Brandon Stone, MD, Victor S. Mangona, MD, Matthew D

APLCC ORAL ABSTRACT SESSIONS - MONDAY, NOVEMBER 26

Aldehyde Dehydrogenase 1A1 Possesses Stem-Like Properties and Predicts Lung Cancer Patient Outcome Xiao Li, MD, Liyan Wan, MD, Jian Geng, MD, Chin-Lee.

Combined Radiotherapy and Anti–PD-L1 Antibody Synergistically Enhances Antitumor Effect in Non–Small Cell Lung Cancer Xiaomei Gong, MD, PhD, Xuefei Li,

Alpha-Actinin 4 Is Associated with Cancer Cell Motility and Is a Potential Biomarker in Non–Small Cell Lung Cancer Ming-Chuan Wang, PhD, Ying-Hua Chang,

EIF4E Is an Adverse Prognostic Marker of Melanoma Patient Survival by Increasing Melanoma Cell Invasion Shahram Khosravi, Kevin J. Tam, Gholamreza S.

A Case of a Patient with Idiopathic Pulmonary Fibrosis with Lung Squamous Cell Carcinoma Treated with Nivolumab Monica Khunger, MD Journal of Thoracic.

The C-terminus of Hsp70-Interacting Protein Promotes Met Receptor Degradation Kang Won Jang, PhD, Jeong Eun Lee, MD, Sun Young Kim, MD, Min-Woong Kang,

Chandra Goparaju, PhD, Jessica S

Ellen M. Schultz, MS, Gerard A. Silvestri, MD, MS, Michael K

Co-Overexpression of Cyclooxygenase-2 and Microsomal Prostaglandin E Synthase-1 Adversely Affects the Postoperative Survival in Non-small Cell Lung Cancer

Electronic Updates for JTO Readers

Nicotine Induces Resistance to Epidermal Growth Factor Receptor Tyrosine Kinase Inhibitor by α1 Nicotinic Acetylcholine Receptor–Mediated Activation in.

Molecular Therapy - Nucleic Acids

Volume 25, Issue 3, Pages (March 2017)

MicroRNA-101 Exerts Tumor-Suppressive Functions in Non-small Cell Lung Cancer through Directly Targeting Enhancer of Zeste Homolog 2 Ji-guang Zhang,

G-Protein Coupled Receptor Family C, Group 5, Member A (gprc5a) Expression Is Decreased in the Adjacent Field and Normal Bronchial Epithelia of Patients.

Decreased expression of FOXA2 promotes eutopic endometrial cell proliferation and migration in patients with endometriosis Anping Lin, Juan Yin, Chao.

Prospective Study on Functional Results After Lung-Sparing Radical Pleurectomy in the Management of Malignant Pleural Mesothelioma Servet Bölükbas, MD,

Pathobiological Implications of MUC4 in Non–Small-Cell Lung Cancer

Socioeconomic Status Is Associated with Depressive Severity Among Patients with Advanced Non–Small-Cell Lung Cancer: Treatment Setting and Minority Status.

miR-124 Inhibits Lung Tumorigenesis Induced by K-ras Mutation and NNK

Yabin Cheng, Guangdi Chen, Magdalena Martinka, Vincent Ho, Gang Li

Relationship Between Functional Preservation after Segmentectomy and Volume- Reduction Effects after Lobectomy in Stage I Non-small Cell Lung Cancer Patients.

Kun-Peng Zhu, Xiao-Long Ma, Chun-Lin Zhang Molecular Therapy

Erratum Journal of Thoracic Oncology

Nicotinamide Phosphoribosyltransferase: A Potent Therapeutic Target in Non-small Cell Lung Cancer with Epidermal Growth Factor Receptor-Gene Mutation

Mitotic Inhibitors Journal of Thoracic Oncology

Figure 1. RSPO3 expression is upregulated in bladder cancer

Figure 1. LOC is highly expressed in NPC and predicts unfavorable prognosis. (A) Differential gene expression ... Figure 1. LOC is highly expressed.

MicroRNA-381 Represses ID1 and is Deregulated in Lung Adenocarcinoma

RhoC Promotes Human Melanoma Invasion in a PI3K/Akt-Dependent Pathway

Long Noncoding RNA BC as a Novel Therapeutic Target for Colorectal Cancer that Suppresses Metastasis by Upregulating TIMP3 Jiaxin Lin, Xin Tan,

Jie Huang, Juan Peng, Linlang Guo, MD, PhD

Molecular Therapy - Nucleic Acids

Elevated Expression of BIRC6 Protein in Non–Small-Cell Lung Cancers is Associated with Cancer Recurrence and Chemoresistance Xin Dong, MD, Dong Lin,

Effects of propofol on the expression of matric metalloproteinases in rat cardiac fibroblasts after hypoxia and reoxygenation J.H. Jun, J.E. Cho, Y.H.

Overexpression of Muscarinic Receptor 3 Promotes Metastasis and Predicts Poor Prognosis in Non–Small-Cell Lung Cancer Gengpeng Lin, MD, Longhua Sun,

APLCC POSTER SESSION 2 - WEDNESDAY, NOVEMBER 28

Molecular Therapy - Oncolytics

Signal Transducer and Activator of Transcription 3 as Molecular Therapy for Non–Small- Cell Lung Cancer Cheng-Yi Wang, MD, Ting-Ting Chao, PhD, Wei-Tien.

Presentation transcript:

Overexpression of Muscarinic Receptor 3 Promotes Metastasis and Predicts Poor Prognosis in Non–Small-Cell Lung Cancer Gengpeng Lin, MD, Longhua Sun, PhD, Ran Wang, PhD, Yubiao Guo, MD, Canmao Xie, MD, PhD Journal of Thoracic Oncology Volume 9, Issue 2, Pages 170-178 (February 2014) DOI: 10.1097/JTO.0000000000000066 Copyright © 2014 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 1 M3R is overexpressed in both NSCLC cell lines and tissues. A Western blot analysis of M3R expression in NSCLC cell lines (GLC82, PC9, SPC-A1, A549, and L78) and HLF. B, Western blot analysis of M3R protein level in 10 pairs of tissues (T: tumor, N: adjacent noncancerous lung tissue). β-actin was used as a loading control. M3R, muscarinic receptor 3; NSCLC, non–small-cell lung cancer; and HLF. Journal of Thoracic Oncology 2014 9, 170-178DOI: (10.1097/JTO.0000000000000066) Copyright © 2014 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 2 Overexpression of M3R correlates with poor prognosis and metastasis in NSCLC patients. A, Representative immunohistochemistry pictures of M3R expression in different NSCLC subtypes and normal lung tissues. B, Survival curves of NSCLC patients with high or low M3R expression (n =148, p < 0.0001; left panel). Differences within subgroups of NSCLC with COPD (n = 60, p = 0.0003; middle panel) and NSCLC without COPD (n = 88, p = 0.0222; right panel). M3R, muscarinic receptor; 3 NSCLC, non–small-cell lung cancer; COPD, chronic obstructive pulmonary disease. Journal of Thoracic Oncology 2014 9, 170-178DOI: (10.1097/JTO.0000000000000066) Copyright © 2014 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 3 Expression of M3R in NSCLC is associated with airway obstruction and smoking. A, Differences in M3R expression in NSCLC tissues between patients with and without COPD. B, Inverse correlation between M3R expression and FEV1/FVC (r = 0.5057, p < 0.0001). C, Inverse correlation between M3R expression and %FEV1 (r = 0.7017, p < 0.0001). D, M3R expression levels in NSCLC patients with and without a smoking history. E, Correlation between M3R expression and smoking (pack-years) (r = 0.3169, p = 0.0014). M3R, muscarinic receptor 3; NSCLC, non–small-cell lung cancer; FEV1, forced expiratory volume in 1 second; FVC, forced vital capacity; COPD, chronic obstructive pulmonary disease. Journal of Thoracic Oncology 2014 9, 170-178DOI: (10.1097/JTO.0000000000000066) Copyright © 2014 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 4 M3R promotes proliferation, migration, and invasion of non–small-cell lung cancer cell lines. A, Knockdown efficiency of M3R siRNA in A549 and L78 cells. B, Growth curves of L78 and A549 after M3R siRNA treatment. A small reduction of absorbance was observed after siRNA treatment (p < 0.01). C, Wound-healing assay to analyze cell migration after M3R siRNA treatment. D, Migration and invasion assay using a transwell system (representative images of migration chambers; average counts from 5 random microscopic fields). M3R siRNA inhibited the migration and invasion ability of L78 and A549. M3R, muscarinic receptor 3. Journal of Thoracic Oncology 2014 9, 170-178DOI: (10.1097/JTO.0000000000000066) Copyright © 2014 International Association for the Study of Lung Cancer Terms and Conditions

FIGURE 5 M3R promotes the migration and invasion of non–small-cell lung cancer cell lines through the PI3K/Akt/MMP9 pathway. A, Zymography of MMP9 in conditioned medium of cells treated with M3R siRNA. B and D, Western bot assay of p-Akt, Akt, E-cadherin, and MMP9 in indicated cells. B, Knockdown of M3R results in the reduced expression of p-Akt and MMP9, but elevated expression of E-cadherin. D, Activation of M3R by Ach induces expression of MMP9 and p-Akt, but reduces expression of E-cadherin. PI3K inhibitor abolishes the effect of Ach. C, Zymography of MMP9 using conditioned medium from cells treated with Ach and LY294002. E, Wound-healing assay for cells migration ability after Ach and LY294002 treatment. A concentration of 10−4M Ach promotes migration, but migration is abolished by LY294002. F, Migration and invasion assay using a transwell system (representative images of migration chambers; average counts from 5 random microscopic fields). Cells were treated with 10-4M Ach with and without 20μM LY294002, respectively. A concentration of 10-4M Ach promotes migration while LY294002 treatment inhibits it. M3R, muscarinic receptor 3, Ach, acetylcholine; MMP9, matrix metalloproteinase 9; NC, negative control; pAKT, phosphor-AKT. Journal of Thoracic Oncology 2014 9, 170-178DOI: (10.1097/JTO.0000000000000066) Copyright © 2014 International Association for the Study of Lung Cancer Terms and Conditions