M. Akagi, M. D. , Ph. D. , A. Ueda, M. D. , T. Teramura, Ph. D. , S

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Oxidized LDL binding to LOX-1 enhances MCP-1 expression in cultured human articular chondrocytes M. Akagi, M.D., Ph.D., A. Ueda, M.D., T. Teramura, Ph.D., S. Kanata, M.D., T. Sawamura, M.D., Ph.D., C. Hamanishi, M.D., Ph.D. Osteoarthritis and Cartilage Volume 17, Issue 2, Pages 271-275 (February 2009) DOI: 10.1016/j.joca.2008.06.019 Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 1 Time and dose effects of ox-LDL on MCP-1 mRNA expression. HACs were incubated with 50g/ml IL-1β, 50μg/ml ox-LDL, or 50μg/ml n-LDL, and MCP-1 mRNA expression was investigated by Real time PCR at the indicated times (A). HACs were incubated with the indicated concentration of IL-1β, n-LDL or ox-LDL for 12h, and MCP-1 mRNA expression was investigated by Real time PCR (B). Cells preincubated with 40μg/ml anti-human LOX-1 mAb (TS92) or non-specific IgG for 30min were also stimulated with ox-LDL. Relative quantity of MCP-1 mRNA was calculated by the delta–delta Ct method. Error bars indicate SDs (n=3). Osteoarthritis and Cartilage 2009 17, 271-275DOI: (10.1016/j.joca.2008.06.019) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions

Fig. 2 Time and dose effects of ox-LDL on MCP-1 protein level. MCP-1 protein level was determined by ELISA of conditioned medium. Time dependent increases in MCP-1 level were observed when cells were incubated with 50pg/ml IL-1β or 50μg/ml ox-LDL (A). HACs were incubated with the indicated concentration of IL-1β, n-LDL or ox-LDL for 24h (B). Cells preincubated with anti-human LOX-1 mAb (TS92) or non-specific IgG for 30min were also stimulated with ox-LDL. Error bars indicate SDs (n=5). Osteoarthritis and Cartilage 2009 17, 271-275DOI: (10.1016/j.joca.2008.06.019) Copyright © 2008 Osteoarthritis Research Society International Terms and Conditions