Do Now: Label the following: Sense strand Anti-sense strand 5’ end of RNA 3’ end of RNA IB Topic 7.4- Translation
Structure of tRNA Clover-shaped Anticodon on one end CCA on the other end, where amino acid will bind.
Hooking the amino acid onto tRNA Involves a tRNA activating enzyme, and uses ATP for energy. Involves a Condensation reaction
Degeneracy of amino acids Some amino acids have more than one mRNA associated with them.
Ribosome structure Made up of protein and rRNA Large subunit- made up of two molecules of rRNA and some proteins 2 binding sites for tRNA (A site and P site) Small subunit- made up of one molecule of rRNA and some proteins Binding site for mRNA
IB Topic 7.4- Translation Binding Sites A site: hold a tRNA carrying the next amino acid to be added to chain P site: hold the tRNA carrying the growing polypeptide chain E site: exit site
Other Translation terms to know Polysomes Ribosomes that are clustered together on the mRNA, creating several copies of the same polypeptide at the same time Start codons Found at the 5’ end of mRNA AUG Stop codons Translation stops- A site is not copied UAG, UAA, UGA
Direction of Translation Occurs in 5’3’ direction with regard to the mRNA. Because start codon is found on the 5’ end
Steps of Translation Initiation Elongation Termination Translocation: mRNA , the first tRNA, and the ribosomal subunits come together Elongation A succession of tRNA’s add their amino acids to the polypeptide chain as mRNA is moved through the ribosome, one codon at a time Termination Ribosome recognizes the stop codon and polypeptide is terminated and release Translocation: Moving from one spot to the other. Animation
Free vs. Bound Ribosomes IB Topic 7.4- Translation Free vs. Bound Ribosomes Free ribosomes- synthesize proteins for use within the cell Bound ribosomes- (rough er) synthesize proteins for secretion or for lysosomes.